Source:http://linkedlifedata.com/resource/pubmed/id/17111193
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
|
pubmed:dateCreated |
2007-1-22
|
pubmed:abstractText |
Brd2 is a novel protein kinase and plays a role in cell cycle-responsive transcription. Recent studies show that Brd2 contributes to E2F-1 regulated cell cycle progression. In this process, Brd2 exhibits scaffold or transcriptional adapter functions and mediates recruitment of both E2F-1 transcription factors and chromatin-remodelling activity to the E2F-1-resposive promoter. In the present study, we show that Brd2 is also a TBP-associated protein and a 26 amino acids peptide in the first bromodomain of Brd2 is essential for Brd2-TBP interaction. We found that serum stimulation of serum starved NIH/3T3 cells efficiently induces the formation of the Brd2-E2F-1-TBP complex in vivo. In this process, Brd2 plays a pivotal role in the recruitment of TBP into a E2F-1 transcriptional complex, as tested in overexpression assay and at the endogenous level. Furthermore, the 26 amino acid peptide that mediates Brd2-TBP interaction is proved to be critical for Brd2-dependent transactivation on E2F-1-responsive promoters, and moreover, Brd2 and E2F-1 may cooperatively participate in various serum-induced transactivation processes in Luciferase-reporter assays. Thus taken together, because Brd2 may recruit a HAT in its transactivational complex and E2F-1 has been found to stimulate transcription by recruiting acetyltransferase and cofactors GCN5, we predict that Brd2 and E2F-1 may act in a cooperative way to introduce an optimal environment for TBP binding to the TATA-element of gene promoters.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Brd2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/E2F1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Box Binding Protein
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0300-8177
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
294
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
45-54
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:17111193-Animals,
pubmed-meshheading:17111193-E2F1 Transcription Factor,
pubmed-meshheading:17111193-Gene Deletion,
pubmed-meshheading:17111193-Immunoblotting,
pubmed-meshheading:17111193-Mice,
pubmed-meshheading:17111193-NIH 3T3 Cells,
pubmed-meshheading:17111193-Precipitin Tests,
pubmed-meshheading:17111193-Protein-Serine-Threonine Kinases,
pubmed-meshheading:17111193-RNA Interference,
pubmed-meshheading:17111193-Recombinant Fusion Proteins,
pubmed-meshheading:17111193-Serum,
pubmed-meshheading:17111193-TATA-Box Binding Protein,
pubmed-meshheading:17111193-Transcriptional Activation,
pubmed-meshheading:17111193-Transfection,
pubmed-meshheading:17111193-Transformation, Genetic
|
pubmed:year |
2007
|
pubmed:articleTitle |
Brd2 is a TBP-associated protein and recruits TBP into E2F-1 transcriptional complex in response to serum stimulation.
|
pubmed:affiliation |
National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|