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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2007-2-26
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pubmed:abstractText |
Female differentiation of Drosophila germ cells is induced by cell-nonautonomous signals generated in the gonadal soma that work with germ-cell-autonomous signals determined by germ-cell X chromosome dose. Generation of the nonautonomous feminizing signals was known to involve female-specific protein encoded by the master sex-determination gene Sex-lethal (Sxl) acting on its switch-gene target transformer (tra) to produce Tra(F) protein. However, it was not known whether Sxl's action on tra alone would suffice to trigger a fully feminizing nonautonomous signal. We developed a constitutively feminizing tra transgene that allowed us to answer this question. In gynanders (XX//XO mosaics) feminized by this Tra(F) transgene, functionally Sxl- haplo-X (chromosomally male) somatic cells collaborated successfully with diplo-X (chromosomally female) germ cells to make functional eggs. The fertility of such gynanders shows not only that Tra(F) is sufficient to elicit a fully feminizing nonautonomous signal, but also that haplo-X somatic cells can execute all other somatic functions required for oogenesis, despite the fact that their genome is not expected to be dosage compensated for such diplo-X-specific functions. The unexpected observation that some Tra(F)-feminized gynanders failed to lay their eggs showed there to be diplo-X cells outside the gonad for which Tra(F)-feminized haplo-X cells cannot substitute.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-10588726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-10652515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-10924471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-11118879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-116894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-12211627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-13481286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-1425338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-15459096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-15762846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-15943970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-16049490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-1909423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-2454747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-2479546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-2612376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-2702687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-3085954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-3920120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-4568399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-5780530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-628449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-6735170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-7054003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-7649398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-7713421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-8076518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-812741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-8162863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-827461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-8601491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-8982468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-9023356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-947995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-9531538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-9622631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17110478-9799737
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
631-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17110478-Animals,
pubmed-meshheading:17110478-Chromosomes,
pubmed-meshheading:17110478-Drosophila Proteins,
pubmed-meshheading:17110478-Drosophila melanogaster,
pubmed-meshheading:17110478-Female,
pubmed-meshheading:17110478-Feminization,
pubmed-meshheading:17110478-Fertility,
pubmed-meshheading:17110478-Genotype,
pubmed-meshheading:17110478-Germ Cells,
pubmed-meshheading:17110478-Gonads,
pubmed-meshheading:17110478-Haploidy,
pubmed-meshheading:17110478-Male,
pubmed-meshheading:17110478-Nuclear Proteins,
pubmed-meshheading:17110478-Oogenesis,
pubmed-meshheading:17110478-Ovum,
pubmed-meshheading:17110478-RNA-Binding Proteins,
pubmed-meshheading:17110478-Transgenes
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pubmed:year |
2007
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pubmed:articleTitle |
Drosophila melanogaster male somatic cells feminized solely by TraF can collaborate with female germ cells to make functional eggs.
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pubmed:affiliation |
Division of Genetics, Genomics and Development, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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