|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2007-2-12
|
|
pubmed:abstractText |
The aim of this study was to investigate the effects of apelin on proliferation and apoptosis of mouse osteoblastic MC3T3-E1 cells. APJ was expressed in MC3T3-E1 cells. Apelin did not affect Runx2 expression, alkaline phosphatase (ALP) activity, osteocalcin and type I collagen secretion, suggesting that it has no effect on osteoblastic differentiation of MC3T3-E1 cells. However, apelin stimulated MC3T3-E1 cell proliferation and inhibited cell apoptosis induced by serum deprivation. Our study also shows that apelin decreased cytochrome c release and caspase-3, capase-8 and caspase-9 activation in serum-deprived MC3T3-E1 cells. Apelin activated c-Jun N-terminal kinase (JNK) and Akt (phosphatidylinositol 3-kinase downstream effector), and the JNK inhibitor SP600125, the phosphatidylinositol 3-kinase (PI3-K) inhibitor LY294002 or the Akt inhibitor 1L-6-hydroxymethyl-chiro-inositol 2-(R)-2-O-methyl-3-O-octadecylcarbonate (HIMO) inhibited its effects on proliferation and serum deprivation-induced apoptosis. Furthermore, apelin protected against apoptosis induced by the glucocorticoid dexamethasone or TNF-alpha. Apelin stimulates proliferation and suppresses serum deprivation-induced apoptosis of MC3T3-E1 cells and these actions are mediated via JNK and PI3-K/Akt signaling pathways.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apln protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Aplnr protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Core Binding Factor Alpha 1 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Runx2 protein, mouse
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0196-9781
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
28
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
708-18
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:17109997-3T3 Cells,
pubmed-meshheading:17109997-Animals,
pubmed-meshheading:17109997-Apoptosis,
pubmed-meshheading:17109997-Carrier Proteins,
pubmed-meshheading:17109997-Caspases,
pubmed-meshheading:17109997-Cell Differentiation,
pubmed-meshheading:17109997-Cell Proliferation,
pubmed-meshheading:17109997-Core Binding Factor Alpha 1 Subunit,
pubmed-meshheading:17109997-Cytochromes c,
pubmed-meshheading:17109997-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:17109997-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:17109997-Mice,
pubmed-meshheading:17109997-Osteoblasts,
pubmed-meshheading:17109997-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:17109997-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:17109997-RNA, Messenger,
pubmed-meshheading:17109997-Receptors, G-Protein-Coupled,
pubmed-meshheading:17109997-Signal Transduction
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Apelin stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 via JNK and PI3-K/Akt signaling pathways.
|
|
pubmed:affiliation |
The School of Nursing of Central South University, 172# Tongzipo Road, Changsha, Hunan 410013, PR China. tangsyuan@yahoo.com.cn
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
