| pubmed-article:17107343 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0288422 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C1335136 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C1335137 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C1335086 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C1335087 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0243125 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0043016 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C2700289 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:17107343 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:17107343 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:17107343 | pubmed:dateCreated | 2007-2-13 | lld:pubmed |
| pubmed-article:17107343 | pubmed:abstractText | Orc5p is one of six subunits constituting the ORC (origin recognition complex), a possible initiator of chromosomal DNA replication in eukaryotes. Orc5p contains a Walker A motif. We recently reported that a strain of Saccharomyces cerevisiae having a mutation in Orc5p's Walker A motif (orc5-A), showed cell-cycle arrest at G2/M and degradation of ORC at high temperatures (37 degrees C). Over-production of Orc4p, another subunit of ORC, specifically suppressed these phenotypes [Takahashi, Yamaguchi, Yamairi, Makise, Takenaka, Tsuchiya and Mizushima (2004) J. Biol. Chem. 279, 8469-8477]. In the present study, we examined the mechanisms of ORC degradation and of its suppression by Orc4p over-production. In orc5-A, at high temperatures, ORC is degraded by proteasomes; either addition of a proteasome inhibitor, or introduction of a mutation of either tan1-1 or nob1-4 that inhibits proteasomes, prevented ORC degradation. Introduction of the tan1-1 mutation restored cell cycle progression, suggesting that the defect was due to ORC degradation by proteasomes. Yeast two-hybrid and co-immunoprecipitation analyses suggested that Orc5p interacts preferentially with Orc4p and that the orc5-A mutation diminishes this interaction. We suggest that this interaction is mediated by the C-terminal region of Orc4p, and the N-terminal region of Orc5p. Based on these observations, we consider that ATP binding to Orc5p is required for efficient interaction with Orc4p and that, in orc5-A, loss of this interaction at higher temperatures allows proteasomes to degrade ORC, causing growth defects. This model could also explain why over-production of Orc4p suppresses the orc5-A strain's phenotype. | lld:pubmed |
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| pubmed-article:17107343 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:17107343 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17107343 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17107343 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:17107343 | pubmed:issn | 1470-8728 | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:TsuchiyaTomof... | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:MizushimaTohr... | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:TakahashiNaok... | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:MakiseMasakiM | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:SuzukiKeitaro... | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:YamairiFumiko... | lld:pubmed |
| pubmed-article:17107343 | pubmed:author | pubmed-author:MatsudaKazuya... | lld:pubmed |
| pubmed-article:17107343 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:17107343 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:17107343 | pubmed:volume | 402 | lld:pubmed |
| pubmed-article:17107343 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17107343 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17107343 | pubmed:pagination | 397-403 | lld:pubmed |
| pubmed-article:17107343 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:17107343 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17107343 | pubmed:articleTitle | Mechanism for the degradation of origin recognition complex containing Orc5p with a defective Walker A motif and its suppression by over-production of Orc4p in yeast cells. | lld:pubmed |
| pubmed-article:17107343 | pubmed:affiliation | Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan. | lld:pubmed |
| pubmed-article:17107343 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17107343 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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