Linked Life Data

17106685

Source:http://linkedlifedata.com/resource/pubmed/id/17106685

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-3-16
pubmed:abstractText
Proline dehydrogenase is the rate-limiting enzyme in proline degradation and serves important functions in the stress responses and development of plants. We isolated two tobacco proline dehydrogenases, NtPDH1 and NtPDH2, in the course of screening for genes upregulated in stressed tobacco (Nicotiana tabacum) microspores. Expression analysis revealed that the two genes are differentially regulated. Under unstressed conditions, their steady-state transcript levels were similar in mature pollen and apical meristems, whereas NtPDH2 was expressed predominantly in vegetative organs, styles, and ovules. The expression of NtPDH1 was maintained at a constant low level during 24 h of dehydration, whereas NtPDH2 was upregulated within 1 h after the onset of stress and subsequently downregulated to undetectable levels. Differential and sustained expression was also found for the two enzymatic isoforms of Arabidopsis thaliana AtPDH. Silencing of the NtPDH genes by RNA interference using the CaMV 35S promoter led to increased proline contents, decreased seed set, delayed seed germination and retarded seedling development pointing towards an important function of at least one of the two NtPDH genes during plant reproductive development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0032-0935
pubmed:author
pubmed:issnType
Print
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1313-24
pubmed:meshHeading
pubmed-meshheading:17106685-Amino Acid Sequence, pubmed-meshheading:17106685-Conserved Sequence, pubmed-meshheading:17106685-DNA, Complementary, pubmed-meshheading:17106685-DNA, Plant, pubmed-meshheading:17106685-Gene Expression Regulation, Developmental, pubmed-meshheading:17106685-Gene Expression Regulation, Enzymologic, pubmed-meshheading:17106685-Gene Expression Regulation, Plant, pubmed-meshheading:17106685-Molecular Sequence Data, pubmed-meshheading:17106685-Plant Proteins, pubmed-meshheading:17106685-Proline Oxidase, pubmed-meshheading:17106685-RNA, Plant, pubmed-meshheading:17106685-RNA Interference, pubmed-meshheading:17106685-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17106685-Sequence Alignment, pubmed-meshheading:17106685-Sequence Homology, Amino Acid, pubmed-meshheading:17106685-Tobacco
pubmed:year
2007
pubmed:articleTitle
Two tobacco proline dehydrogenases are differentially regulated and play a role in early plant development.
pubmed:affiliation
Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, University of Vienna, Dr. Bohrgasse 9, 1030 Wien, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't