|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
10
|
|
pubmed:dateCreated |
1991-7-11
|
|
pubmed:abstractText |
We describe the synthesis of 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides and demonstrate their utility as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. These 2'-O-modified compounds were designed to possess the binding affinity of an RNA molecule towards a complementary RNA target with an enhanced stability against nucleases. The 2'-O-methyl and 2'-O-ethyl antisense compounds function as potent inhibitors of the reaction at 1-10 nM, approximately 5-fold more effective than a natural antisense RNA molecule and requiring an approximate 5-fold excess over the target RNA for 80% inhibition of the processing reaction.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-1696685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-1824936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2145581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2147994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2170025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2308835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2402441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2434240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2438160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2438655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2478298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2479828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2526684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2531659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2580642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2684648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2726482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2726730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2734288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2825355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2835659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-2962194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3015597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3022153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3073387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3203381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3375062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3380087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3396543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3627981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-3737406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-4088863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-6084590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1710340-6828386
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
25
|
|
pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2629-35
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:1710340-Base Sequence,
pubmed-meshheading:1710340-Chromatography, High Pressure Liquid,
pubmed-meshheading:1710340-Histones,
pubmed-meshheading:1710340-Methylation,
pubmed-meshheading:1710340-Molecular Sequence Data,
pubmed-meshheading:1710340-Nucleic Acid Conformation,
pubmed-meshheading:1710340-Oligodeoxyribonucleotides,
pubmed-meshheading:1710340-Oligoribonucleotides,
pubmed-meshheading:1710340-RNA,
pubmed-meshheading:1710340-RNA, Antisense,
pubmed-meshheading:1710340-RNA Processing, Post-Transcriptional,
pubmed-meshheading:1710340-Ribonucleoproteins,
pubmed-meshheading:1710340-Ribonucleoproteins, Small Nuclear,
pubmed-meshheading:1710340-Thionucleotides
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event.
|
|
pubmed:affiliation |
Institute for Molecular Pathology, Vienna, Austria.
|
|
pubmed:publicationType |
Journal Article
|
