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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2006-11-22
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pubmed:abstractText |
DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methylation assays on bisulphite modified DNA heralded a breakthrough in speed and sensitivity for gene methylation analysis. Despite this technological advancement, these approaches require a cumbersome gene by gene primer design and experimental validation. Bisulphite DNA modification results in sequence alterations (all unmethylated cytosines are converted into uracils) and a general sequence complexity reduction as cytosines become underrepresented. Consequently, standard BLAST sequence homology searches cannot be applied to search for specific methylation primers.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-10471384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-10766168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-11782440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-12042769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-12238773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-12671664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-12758266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-1542678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-15608243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-15608244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-15608257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-16369569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-16381837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-16381876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-16381933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-16381959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-8790415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-9171109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-9171110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17094804-9892187
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2105
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
496
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17094804-Algorithms,
pubmed-meshheading:17094804-Animals,
pubmed-meshheading:17094804-Computational Biology,
pubmed-meshheading:17094804-DNA Methylation,
pubmed-meshheading:17094804-DNA Primers,
pubmed-meshheading:17094804-Databases, Genetic,
pubmed-meshheading:17094804-Databases, Protein,
pubmed-meshheading:17094804-Epigenesis, Genetic,
pubmed-meshheading:17094804-Humans,
pubmed-meshheading:17094804-Internet,
pubmed-meshheading:17094804-Mice,
pubmed-meshheading:17094804-Polymerase Chain Reaction,
pubmed-meshheading:17094804-Rats,
pubmed-meshheading:17094804-Software,
pubmed-meshheading:17094804-Sulfites
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pubmed:year |
2006
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pubmed:articleTitle |
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis.
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pubmed:affiliation |
Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium. Filip.Pattyn@UGent.be <Filip.Pattyn@UGent.be>
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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