Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2006-12-6
pubmed:abstractText
HIV-1 integrase (IN) is the key enzyme catalyzing the proviral DNA integration step. Although the enzyme catalyzes the integration step accurately in vitro, whether IN is sufficient for in vivo integration and how it interacts with the cellular machinery remains unclear. We set up a yeast cellular integration system where integrase was expressed as the sole HIV-1 protein and targeted the chromosomes. In this simple eukaryotic model, integrase is necessary and sufficient for the insertion of a DNA containing viral LTRs into the genome, thereby allowing the study of the isolated integration step independently of other viral mechanisms. Furthermore, the yeast system was used to identify cellular mechanisms involved in the integration step and allowed us to show the role of homologous recombination systems. We demonstrated physical interactions between HIV-1 IN and RAD51 protein and showed that HIV-1 integrase activity could be inhibited both in the cell and in vitro by RAD51 protein. Our data allowed the identification of RAD51 as a novel in vitro IN cofactor able to down regulate the activity of this retroviral enzyme, thereby acting as a potential cellular restriction factor to HIV infection.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-10213687, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-10655210, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-10656788, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-10773452, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11006285, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11070016, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11102318, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11248084, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11346660, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11406603, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11430827, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11479624, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11689615, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-11884535, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12016221, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12020817, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12060741, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12370191, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12407101, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12663813, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12796494, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-12811817, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-14628682, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-14644507, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-14999095, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15297876, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15312229, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15629457, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15718297, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15855167, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15956566, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-15958388, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-16388992, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-1735128, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-2578227, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-7801128, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-8662188, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-9038339, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-9499023, http://linkedlifedata.com/resource/pubmed/commentcorrection/17090598-9860958
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6215-24
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51.
pubmed:affiliation
UMR 5097-CNRS, Bordeaux, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural