Linked Life Data

17089718

Source:http://linkedlifedata.com/resource/pubmed/id/17089718

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-11-8
pubmed:abstractText
The toxicity of cadmium, lead, chromium, and arsenite on Caenorhabditis elegans was investigated to identify sensitive biomarkers for environmental monitoring and risk assessment. Effects of these metals on stress-related gene expression, growth, reproduction, and mortality of C. elegans were investigated under laboratory conditions. The possibility of using C. elegans as a biosensor for environmental toxicity monitoring was also tested using a green fluorescent protein transgenic nematode. The 24-h median lethal concentrations of cadmium, lead, chromium, and arsenite in C. elegans were 846, 34, 115, and 92 mg/L, respectively. Cadmium exposure led to an increase in the expression of most of the genes tested. The degree of increase was more than threefold compared to control in heat shock protein 16.2, heat shock protein 70, metallothionein 2, cytochrome P450 family protein 35A2, glutathione-S-transferase 4, superoxide dismutase 1, catalase 2, C. elegans p53-like protein 1, and apoptosis enhancer 1 genes. The lead-, chromium-, and arsenite-exposed nematode, on the other hand, showed little change in gene expression. Alterations in growth and reproduction were observed in cadmium- and chromium-exposed worms. To consider a transgenic nematode as a biosensor for toxicity monitoring, the responses of stress-related gene promoters need to be tested with a variety of metals. The overall results suggest that cadmium exhibits a high level of tolerance compared to the other metals tested. Use of the responses of stress-related gene expression therefore has considerable potential as a sensitive biomarker for the diagnosis of cadmium contamination, and C. elegans seems to be a good biological model for this approach.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0730-7268
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2946-56
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Assessment of stress-related gene expression in the heavy metal-exposed nematode Caenorhabditis elegans: a potential biomarker for metal-induced toxicity monitoring and environmental risk assessment.
pubmed:affiliation
Faculty of Environmental Engineering, College of Urban Science, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't