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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-1-11
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pubmed:abstractText |
We formulate a general analysis to determine the two-dimensional dissociation constant (2D Kd), and use this method to study the interaction of CD2-expressing T cells with glass-supported planar bilayers containing fluorescently labeled CD58, a CD2 counter-receptor. Both CD2 and CD58 are laterally mobile in their respective membranes. Adhesion is indicated by accumulation of CD2 and CD58 in the cell-bilayer contact area; adhesion molecule density and contact area size attain equilibrium within 40 min. The standard (Scatchard) analysis of solution-phase binding is not applicable to the case of laterally mobile adhesion molecules due to the dynamic nature of the interaction. We derive a new binding equation, B/F=[(Ntxf)/(KdxScell)]-[(Bxp)/Kd], where B and F are bound and free CD58 density in the contact area, respectively; Nt is CD2 molecule number per cell; f is CD2 fractional mobility; Scell is cell surface area; and p is the ratio of contact area at equilibrium to Scell. We use this analysis to determine that the 2D Kd for CD2-CD58 is 5.4-7.6 molecules/microm2. 2D Kd analysis provides a general and quantitative measure of the mechanisms regulating cell-cell adhesion.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17085486-10049348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17085486-10723794,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17085486-9700513
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1022-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17085486-Antigens, CD2,
pubmed-meshheading:17085486-Antigens, CD58,
pubmed-meshheading:17085486-Cell Adhesion Molecules,
pubmed-meshheading:17085486-Humans,
pubmed-meshheading:17085486-Jurkat Cells,
pubmed-meshheading:17085486-Kinetics,
pubmed-meshheading:17085486-Microscopy, Fluorescence,
pubmed-meshheading:17085486-Protein Binding,
pubmed-meshheading:17085486-Protein Interaction Mapping
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pubmed:year |
2007
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pubmed:articleTitle |
Analysis of two-dimensional dissociation constant of laterally mobile cell adhesion molecules.
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pubmed:affiliation |
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Hematology Division, Brigham and Women's Hospital, Boston, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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