Linked Life Data

17085091

Source:http://linkedlifedata.com/resource/pubmed/id/17085091

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-2-6
pubmed:abstractText
During long bone development, cartilage replacement by bone is governed in part by angiogenesis. Although it has been demonstrated that vascular endothelial growth factor (VEGF-A) is crucial during endochondral ossification, little is known about the involvement of the other VEGF family members. Thus, we examined the expression and production of these members on primary chondrocytes and ATDC5 chondrogenic cells. VEGF-A, VEGF-B, VEGF-C and VEGF-D were shown to be expressed and synthesized demonstrating that numerous angiogenic factors can be produced by chondrocytes. In ATDC5 VEGF-A, VEGF-B and VEGF-C were over-expressed in the presence of chondrogenic and bone morphogenetic protein (BMP)-2 treatment suggesting that these factors play an important role during chondrogenesis. In addition, neuropilin-1, VEGF receptor-2 and VEGF receptor-3 gene expression were observed with an increase in VEGF-R2 expression under chondrogenic and BMP-2 treatment, suggesting that VEGF proteins could act in an autocrine/paracrine manner in addition to their angiogenic function. In conclusion, we demonstrated for the first time that chondrocytes secreted the four members of the VEGF family. We also showed that VEGF-B, VEGF-C and VEGF-D were secreted as processed proteins. The up-regulation of VEGF-B and VEGF-C at the mRNA and protein levels under chondrogenic stimulation strongly suggests a major role for these proteins in growth plate physiology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
8756-3282
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
568-76
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17085091-Animals, pubmed-meshheading:17085091-Blotting, Western, pubmed-meshheading:17085091-Bone Morphogenetic Protein 2, pubmed-meshheading:17085091-Bone Morphogenetic Proteins, pubmed-meshheading:17085091-Cell Differentiation, pubmed-meshheading:17085091-Chondrocytes, pubmed-meshheading:17085091-Gene Expression, pubmed-meshheading:17085091-Gene Expression Regulation, Developmental, pubmed-meshheading:17085091-Growth Plate, pubmed-meshheading:17085091-Humans, pubmed-meshheading:17085091-Osteogenesis, pubmed-meshheading:17085091-Protein Isoforms, pubmed-meshheading:17085091-RNA, Messenger, pubmed-meshheading:17085091-Receptors, Vascular Endothelial Growth Factor, pubmed-meshheading:17085091-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17085091-Transforming Growth Factor beta, pubmed-meshheading:17085091-Vascular Endothelial Growth Factor A
pubmed:year
2007
pubmed:articleTitle
VEGF and VEGF receptors are differentially expressed in chondrocytes.
pubmed:affiliation
INSERM UMRS 791, University of Nantes, Laboratoire d'Ingénierie Ostéoarticulaire et Dentaire, LIOAD, School of Dental Surgery, 1 Place Alexis Ricordeau, Nantes Cedex 1, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't