Source:http://linkedlifedata.com/resource/pubmed/id/17082783
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2007-2-14
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pubmed:abstractText |
Hyaluronan (HA), a major component of the cutaneous extracellular-matrix, is involved in tissue repair. Human skin is exposed to and damaged by UVB-irradiation. Here, we investigate the regulation of HA metabolism in human skin during acute UVB-induced inflammation. Expression of HA synthesizing (HAS) and degrading enzymes hyaluronidase (HYAL) as evaluated by quantitative reverse transcribed PCR in response to UVB differed when fibroblasts and HaCaT-keratinocytes, representative cell types in dermis and epidermis, respectively, were compared. Both demonstrated temporally different expression patterns of these genes 3- and 24-hours post-irradiation. This resulted 24-hours post-irradiation in an increase in HAS gene expression in both fibroblasts and HaCaT-keratinocytes, and an increase in HYAL expression only in fibroblasts. HA-production as analyzed by the HA content of conditioned medium was reduced in HaCaT and fibroblast cultures 3-hours post-irradiation, whereas HA increased in HaCaT-cultures 24-hours post-irradiation but remained suppressed in fibroblasts-cultures. Consistently, immunohistochemical staining for HA in human skin 24-hours post-irradiation demonstrated an increased epidermal HA, but a decrease in the dermal compartment. Moreover, analysis of the HA content of dermal microdialysis-fluid revealed increased accumulation of HA degradation products 24-hours post-irradiation. These data demonstrate that there is a complex temporal and spatial regulation of HA-metabolism in skin in response to UVB irradiation.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1523-1747
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
127
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
687-97
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17082783-Cells, Cultured,
pubmed-meshheading:17082783-Dermis,
pubmed-meshheading:17082783-Epidermis,
pubmed-meshheading:17082783-Fibroblasts,
pubmed-meshheading:17082783-Gene Expression Regulation,
pubmed-meshheading:17082783-Humans,
pubmed-meshheading:17082783-Hyaluronic Acid,
pubmed-meshheading:17082783-Keratinocytes,
pubmed-meshheading:17082783-Male,
pubmed-meshheading:17082783-RNA, Messenger,
pubmed-meshheading:17082783-Skin,
pubmed-meshheading:17082783-Skin Physiological Phenomena,
pubmed-meshheading:17082783-Time Factors,
pubmed-meshheading:17082783-Ultraviolet Rays
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pubmed:year |
2007
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pubmed:articleTitle |
Differential regulation of hyaluronan metabolism in the epidermal and dermal compartments of human skin by UVB irradiation.
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pubmed:affiliation |
Department of Dermatology, Venerology and Allergology, Leipzig University Medical Center, Leipzig, Germany. marco.averbeck@medizin.uni-leipzig.de
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pubmed:publicationType |
Journal Article
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