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rdf:type |
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lifeskim:mentions |
umls-concept:C0003316,
umls-concept:C0017349,
umls-concept:C0020792,
umls-concept:C0025202,
umls-concept:C0030705,
umls-concept:C0039194,
umls-concept:C0175631,
umls-concept:C0205369,
umls-concept:C0385723,
umls-concept:C1332714,
umls-concept:C1334510,
umls-concept:C2348480,
umls-concept:C2827662
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pubmed:issue |
10
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pubmed:dateCreated |
2006-11-3
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pubmed:abstractText |
Over the past decade, many efforts have been made to identify MHC class II-restricted epitopes from different tumor-associated Ags. Melan-A/MART-1(26-35) parental or Melan-A/MART-1(26-35(A27L)) analog epitopes have been widely used in melanoma immunotherapy to induce and boost CTL responses, but only one Th epitope is currently known (Melan-A51-73, DRB1*0401 restricted). In this study, we describe two novel Melan-A/MART-1-derived sequences recognized by CD4 T cells from melanoma patients. These epitopes can be mimicked by peptides Melan-A27-40 presented by HLA-DRB1*0101 and HLA-DRB1*0102 and Melan-A25-36 presented by HLA-DQB1*0602 and HLA-DRB1*0301. CD4 T cell clones specific for these epitopes recognize Melan-A/MART-1+ tumor cells and Melan-A/MART-1-transduced EBV-B cells and recognition is reduced by inhibitors of the MHC class II presentation pathway. This suggests that the epitopes are naturally processed and presented by EBV-B cells and melanoma cells. Moreover, Melan-A-specific Abs could be detected in the serum of patients with measurable CD4 T cell responses specific for Melan-A/MART-1. Interestingly, even the short Melan-A/MART-1(26-35(A27L)) peptide was recognized by CD4 T cells from HLA-DQ6+ and HLA-DR3+ melanoma patients. Using Melan-A/MART-1(25-36)/DQ6 tetramers, we could detect Ag-specific CD4 T cells directly ex vivo in circulating lymphocytes of a melanoma patient. Together, these results provide the basis for monitoring of naturally occurring and vaccine-induced Melan-A/MART-1-specific CD4 T cell responses, allowing precise and ex vivo characterization of responding T cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-D Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQ Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQ beta-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQB1 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6769-79
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17082590-Amino Acid Sequence,
pubmed-meshheading:17082590-Antigen Presentation,
pubmed-meshheading:17082590-Antigens, Neoplasm,
pubmed-meshheading:17082590-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17082590-Cancer Vaccines,
pubmed-meshheading:17082590-Cell Separation,
pubmed-meshheading:17082590-Clone Cells,
pubmed-meshheading:17082590-Epitopes, T-Lymphocyte,
pubmed-meshheading:17082590-HLA-D Antigens,
pubmed-meshheading:17082590-HLA-DQ Antigens,
pubmed-meshheading:17082590-HLA-DQ beta-Chains,
pubmed-meshheading:17082590-Humans,
pubmed-meshheading:17082590-MART-1 Antigen,
pubmed-meshheading:17082590-Melanoma,
pubmed-meshheading:17082590-Membrane Glycoproteins,
pubmed-meshheading:17082590-Molecular Sequence Data,
pubmed-meshheading:17082590-Neoplasm Proteins,
pubmed-meshheading:17082590-Peptide Fragments,
pubmed-meshheading:17082590-Signal Transduction
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pubmed:year |
2006
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pubmed:articleTitle |
Melan-A/MART-1-specific CD4 T cells in melanoma patients: identification of new epitopes and ex vivo visualization of specific T cells by MHC class II tetramers.
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pubmed:affiliation |
Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, Lausanne Branch, University Hospital, Lausanne, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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