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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-11-27
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pubmed:abstractText |
In order to develop a fluorescence polarization (FP) assay for calcium binding proteins, a fluorescent peptides based library of 1328 compounds has been synthesized. The use of this library has been validated by setting up a FP-high-throughput screening (FP-HTS) assay for calmodulin using the synthetic gene product (synCaM). With this assay, a set of 880 FDA approved compounds was screened. Besides the promazine class, we discovered two new classes of compounds that interact with calmodulin in a calcium dependent manner. One class has compounds with anti-histaminic/spasmolytic activities, and the other one are detergents with antibacterial activities.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1763
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1250-5
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Use of a fluorescent polarization based high throughput assay to identify new calmodulin ligands.
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pubmed:affiliation |
UMR 7175- LC1, Institut Gilbert Laustriat, Département de Pharmacochimie de la Communication Cellulaire, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Validation Studies
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