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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-3-1
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pubmed:abstractText |
The aim of the present study was to elucidate whether the culture of cells recovered from induced sputum may represent a suitable model to evaluate cytokine and chemokine production by airway inflammatory cells. Sputum induction was performed in 21 normal subjects and 30 asthmatic patients. A total of 21 out of the 30 asthmatic patients were taking inhaled corticosteroids, while the remaining nine were steroid-naive asthmatics. The steroid-naive group was evaluated before and after a 14-day treatment with oral prednisone (40 mg.day(-1)). The supernatant of lysed and centrifuged sputum and the supernatant of sputum cell culture were analysed. Tumour necrosis factor-alpha, interleukin (IL)-8 (CXCL8), IL-1beta, IL-13 and eotaxin-2 (CCL24) concentrations were determined by specific ELISA. Eotaxin-2 production by cell culture was higher in the asthma group (131+/-108 pg.mL(-1)) than in the control group (36+/-41 pg.mL(-1)) and treatment with oral corticosteroids eliminated this difference. In addition, reduction of eotaxin-2 levels by corticosteroid treatment was greater in cell culture (81.3% reduction) than in sputum (26.4%). There was correlation between the decrease in eotaxin-2 production and the decrease in blood eosinophil number and between eotaxin-2 and eosinophils in sputum. Eotaxin-2 may play an important role in asthma and the response to corticosteroid treatment suggests that analysis of sputum cell culture is relevant as an inflammatory parameter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Budesonide,
http://linkedlifedata.com/resource/pubmed/chemical/CCL24 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL24,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Prednisone,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0903-1936
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
489-95
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:17079258-Administration, Inhalation,
pubmed-meshheading:17079258-Administration, Oral,
pubmed-meshheading:17079258-Adult,
pubmed-meshheading:17079258-Anti-Inflammatory Agents,
pubmed-meshheading:17079258-Asthma,
pubmed-meshheading:17079258-Budesonide,
pubmed-meshheading:17079258-Cells, Cultured,
pubmed-meshheading:17079258-Chemokine CCL24,
pubmed-meshheading:17079258-Chemokines,
pubmed-meshheading:17079258-Chemokines, CC,
pubmed-meshheading:17079258-Cytokines,
pubmed-meshheading:17079258-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17079258-Eosinophils,
pubmed-meshheading:17079258-Female,
pubmed-meshheading:17079258-Forced Expiratory Volume,
pubmed-meshheading:17079258-Humans,
pubmed-meshheading:17079258-Interleukin-13,
pubmed-meshheading:17079258-Interleukin-1beta,
pubmed-meshheading:17079258-Interleukin-8,
pubmed-meshheading:17079258-Leukocyte Count,
pubmed-meshheading:17079258-Lipopolysaccharides,
pubmed-meshheading:17079258-Male,
pubmed-meshheading:17079258-Middle Aged,
pubmed-meshheading:17079258-Prednisone,
pubmed-meshheading:17079258-Reference Values,
pubmed-meshheading:17079258-Saliva,
pubmed-meshheading:17079258-Statistics as Topic,
pubmed-meshheading:17079258-Tumor Necrosis Factor-alpha
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pubmed:year |
2007
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pubmed:articleTitle |
Eotaxin-2 in sputum cell culture to evaluate asthma inflammation.
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pubmed:affiliation |
Dept of Medicine, University of S. Paulo Medical School at Ribeirão Preto, Av. Bandeirantes 3900, Ribeirão Preto, Sãu Paulo, 14048-900, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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