Linked Life Data

170791

Source:http://linkedlifedata.com/resource/pubmed/id/170791

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1975-12-30
pubmed:abstractText
A number of different techniques to be used for the subtyping of herpes simplex virus (HSV) strains were studied. The strains were inoculated on chorio-allantoic membranes of embryonated eggs and on green monkey kidney (GMK) cells in order that the morphology of plaques produced might be observed. They were classified serologically by determination of K-values and inoculated intracerebrally in mice in order that their pathogenicity for mice might be observed. The inhibitory effect of high concentrations of thymidine on the multiplication of the strains in GMK cells cultures and the heat-stability of the virus-induced thymidine kinases were investigated. Rates of inactivation of the strains in the presence of AgNO3 were compared and, finally, the association of focal liver necrosis in intraperitoneally inoculated mice with the results of the serological typing was observed. The results suggested that the liver necrosis test was simple as well as accurate and useful as a screening typing-test. Reliable results were also obtained serologically and by the method demonstrating differences in the heat-stability of viral thymidine kinase. Using the other methods studied, difficulties to obtain clear-cut or reproducible typing results were encountered.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0105-0656
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
506-12
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Subtyping of herpes simplex virus.
pubmed:publicationType
Journal Article