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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-11-8
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pubmed:abstractText |
The genetic locus of Nkx3.1, an early murine marker of sclerotome and prostate development, was disrupted by a knock in of CRE recombinase via homologous recombination in embryonic stem cells. Cell fate mapping revealed previously unidentified cell lineages expanded from Nkx3.1-expressing cell populations and recapitulated reported Nkx3.1 expression patterns. In lineage trace experiments of E18.5 Nkx3.1-CRE; R26R embryos novel staining was observed in areas of the lungs, portions of the duodenum, and vertebral elements of the skeleton. beta-galactosidase activity measured in Nkx3.1-CRE; R26R and Nkx3.2-CRE; R26R embryos was observed in overlapping regions of the sclerotome but no apparent change in Nkx3.1 expression was seen in the Nkx3.2 mutants by in situ hybridization.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1526-954X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
550-5
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pubmed:meshHeading |
pubmed-meshheading:17078065-Animals,
pubmed-meshheading:17078065-Cell Differentiation,
pubmed-meshheading:17078065-Cell Lineage,
pubmed-meshheading:17078065-DNA Primers,
pubmed-meshheading:17078065-Duodenum,
pubmed-meshheading:17078065-Embryonic Stem Cells,
pubmed-meshheading:17078065-Gene Expression Profiling,
pubmed-meshheading:17078065-Gene Expression Regulation, Developmental,
pubmed-meshheading:17078065-Homeodomain Proteins,
pubmed-meshheading:17078065-In Situ Hybridization,
pubmed-meshheading:17078065-Lung,
pubmed-meshheading:17078065-Mice,
pubmed-meshheading:17078065-Spine,
pubmed-meshheading:17078065-Transcription Factors,
pubmed-meshheading:17078065-beta-Galactosidase
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pubmed:year |
2006
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pubmed:articleTitle |
Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice.
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pubmed:affiliation |
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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