Linked Life Data

17074903

Source:http://linkedlifedata.com/resource/pubmed/id/17074903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 11
pubmed:dateCreated
2006-10-31
pubmed:databankReference
pubmed:abstractText
Photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, produces a siderophore which is distinct from that produced by P. damselae subsp. damselae. Using suppression subtractive hybridization, a subsp. piscicida-specific DNA region of 35 kb was identified in strain DI21, and 11 genes were defined: dahP, araC1, araC2, frpA, irp8, irp2, irp1, irp3, irp4, irp9 and irp5. The sequence of the predicted proteins encoded by these genes showed significant similarity with the proteins responsible for the synthesis and transport of the siderophore yersiniabactin, encoded within the Yersinia high-pathogenicity island (HPI). Southern hybridization demonstrated that this gene cluster is exclusive to some European subsp. piscicida isolates. Database searches revealed that a similar gene cluster is present in Photobacterium profundum SS9 and Vibrio cholerae RC385. An irp1 gene (encoding a putative non-ribosomal peptide synthetase) insertional mutant (CS31) was impaired for growth under iron-limiting conditions and unable to produce siderophores, and showed an approximately 100-fold decrease in degree of virulence for fish. The subsp. piscicida DI21 strain, but not CS31, promoted the growth of a Yersinia enterocolitica irp1 mutant. Furthermore, a yersiniabactin-producing Y. enterocolitica strain as well as purified yersiniabactin were able to cross-feed strains DI21 and CS31, suggesting that the subsp. piscicida siderophore might be functionally and structurally related to yersiniabactin. The differential occurrence among P. damselae strains, and the low sequence similarity to siderophore synthesis genes described in other members of the Vibrionaceae, suggest that this genetic system might have been acquired by horizontal transfer in P. damselae subsp. piscicida, and might have a common evolutionary origin with the Yersinia HPI.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3327-41
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:17074903-Animals, pubmed-meshheading:17074903-Benzethonium, pubmed-meshheading:17074903-Biological Evolution, pubmed-meshheading:17074903-Fish Diseases, pubmed-meshheading:17074903-Fishes, pubmed-meshheading:17074903-Gene Transfer, Horizontal, pubmed-meshheading:17074903-Genes, Bacterial, pubmed-meshheading:17074903-Genomic Islands, pubmed-meshheading:17074903-Gram-Negative Bacterial Infections, pubmed-meshheading:17074903-Molecular Sequence Data, pubmed-meshheading:17074903-Multigene Family, pubmed-meshheading:17074903-Peptide Synthases, pubmed-meshheading:17074903-Photobacterium, pubmed-meshheading:17074903-Point Mutation, pubmed-meshheading:17074903-Siderophores, pubmed-meshheading:17074903-Virulence, pubmed-meshheading:17074903-Virulence Factors, pubmed-meshheading:17074903-Yersinia
pubmed:year
2006
pubmed:articleTitle
A siderophore biosynthesis gene cluster from the fish pathogen Photobacterium damselae subsp. piscicida is structurally and functionally related to the Yersinia high-pathogenicity island.
pubmed:affiliation
Department of Microbiology and Parasitology, Institute of Aquaculture and Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela 15782, Galicia, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't