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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-1-15
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pubmed:abstractText |
Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells in culture was so far studied only on the RNA-level. We here describe its genomic organisation and detect a new brain expressed (CRTAC1-B) isoform resulting from alternate last exon usage which is highly conserved in vertebrates. In humans, we identify an exon sharing process with the neighbouring tail-to-tail orientated gene leading to CRTAC1-A. This isoform is produced by cultured human chondrocytes, localized in the extracellular matrix of articular cartilage and its secretion can be stimulated by BMP4. Of five putative O-glycosylation motifs in the last exon of CRTAC1-A, the most C-terminal one is modified according to exposure of serial C-terminal deletion mutants to the O-glycosylation inhibitor Benzyl-alpha-GalNAc. Both isoforms contain four FG-GAP repeat domains and an RGD integrin binding motif, suggesting cell-cell or cell-matrix interaction potential. In summary, CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylgalactosamine,
http://linkedlifedata.com/resource/pubmed/chemical/BMP4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Benzyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 4,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/COL2A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CRTAC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Threonine,
http://linkedlifedata.com/resource/pubmed/chemical/benzyl-alpha-N-acetylgalactosamine
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0945-053X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30-41
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pubmed:dateRevised |
2010-5-21
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pubmed:meshHeading |
pubmed-meshheading:17074475-Acetylgalactosamine,
pubmed-meshheading:17074475-Adult,
pubmed-meshheading:17074475-Amino Acid Sequence,
pubmed-meshheading:17074475-Animals,
pubmed-meshheading:17074475-Base Sequence,
pubmed-meshheading:17074475-Benzyl Compounds,
pubmed-meshheading:17074475-Binding Sites,
pubmed-meshheading:17074475-Bone Morphogenetic Protein 4,
pubmed-meshheading:17074475-Bone Morphogenetic Proteins,
pubmed-meshheading:17074475-Brain,
pubmed-meshheading:17074475-Calcium-Binding Proteins,
pubmed-meshheading:17074475-Cartilage, Articular,
pubmed-meshheading:17074475-Child,
pubmed-meshheading:17074475-Chondrocytes,
pubmed-meshheading:17074475-Collagen Type II,
pubmed-meshheading:17074475-Enzyme Inhibitors,
pubmed-meshheading:17074475-Exons,
pubmed-meshheading:17074475-Extracellular Matrix Proteins,
pubmed-meshheading:17074475-Glycosylation,
pubmed-meshheading:17074475-Humans,
pubmed-meshheading:17074475-Lung,
pubmed-meshheading:17074475-Male,
pubmed-meshheading:17074475-Middle Aged,
pubmed-meshheading:17074475-Molecular Sequence Data,
pubmed-meshheading:17074475-Protein Isoforms,
pubmed-meshheading:17074475-Sequence Homology, Amino Acid,
pubmed-meshheading:17074475-Threonine,
pubmed-meshheading:17074475-Vertebrates
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pubmed:year |
2007
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pubmed:articleTitle |
Chondrocyte secreted CRTAC1: a glycosylated extracellular matrix molecule of human articular cartilage.
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pubmed:affiliation |
Division of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Schlierbacher Landstrasse 200a, D-69118 Heidelberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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