Linked Life Data

17072841

Source:http://linkedlifedata.com/resource/pubmed/id/17072841

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-1-15
pubmed:abstractText
Mesenchymal stem cells (MSCs) are a candidate for replacing chondrocytes in cell-based repair of cartilage lesions. However, it has not been clarified if these cells can acquire the hyaline phenotype, and whether chondrocytes and MSCs show the same expression patterns of critical control genes in development. In order to study this, articular chondrocytes and iliac crest derived MSCs were allowed to differentiate in pellet mass cultures. Gene expression of markers for the cartilage phenotype, helix-loop-helix (HLH) transcription factors, and chondrogenic transcription factors were analyzed by real-time PCR. Matrix production was assayed using biochemical analysis for hydroxyproline, glycosaminoglycans, and immunohistochemistry for collagen types I and II. Significantly decreased expression of collagen type I was accompanied by increased expression of collagen types IIA and IIB during differentiation of chondrocytes, indicating differentiation towards a hyaline phenotype. Chondrogenesis in MSCs on the other hand resulted in up-regulation of collagen types I, IIA, IIB, and X, demonstrating differentiation towards cartilage of a mixed phenotype. Expression of HES1 increased significantly during chondrogenesis in chondrocytes while expression in MSCs was maintained at a low level. The HLH gene HES5 on the other hand was only detected in chondrocytes. Expression of ID1 decreased significantly in chondrocytes while the opposite was seen in MSCs. These findings suggest that chondrocytes and MSCs differentiated and formed different subtypes of cartilage, the hyaline and a mixed cartilage phenotype, respectively. Differentially regulated HLH genes indicated the possibility for HLH proteins in regulating chondrogenic differentiation. This information is important to understand the potential use of MSCs in cartilage repair.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0736-0266
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
152-63
pubmed:meshHeading
pubmed-meshheading:17072841-Adult, pubmed-meshheading:17072841-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:17072841-Cell Differentiation, pubmed-meshheading:17072841-Cells, Cultured, pubmed-meshheading:17072841-Chondrocytes, pubmed-meshheading:17072841-Chondrogenesis, pubmed-meshheading:17072841-Collagen Type I, pubmed-meshheading:17072841-Collagen Type X, pubmed-meshheading:17072841-Flow Cytometry, pubmed-meshheading:17072841-Gene Expression Regulation, pubmed-meshheading:17072841-Helix-Loop-Helix Motifs, pubmed-meshheading:17072841-Homeodomain Proteins, pubmed-meshheading:17072841-Humans, pubmed-meshheading:17072841-Immunohistochemistry, pubmed-meshheading:17072841-Inhibitor of Differentiation Protein 1, pubmed-meshheading:17072841-Mesenchymal Stem Cells, pubmed-meshheading:17072841-Middle Aged, pubmed-meshheading:17072841-Repressor Proteins, pubmed-meshheading:17072841-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17072841-Transcription Factors
pubmed:year
2007
pubmed:articleTitle
Differentiation of human mesenchymal stem cells and articular chondrocytes: analysis of chondrogenic potential and expression pattern of differentiation-related transcription factors.
pubmed:affiliation
Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg University, Göteborg, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't