Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-5-6
pubmed:abstractText
Vascular cell adhesion molecule 1 (VCAM-1)/inducible cell adhesion molecule 110 is a mononuclear leukocyte-selective adhesion molecule, expressed on vascular endothelium following activation by certain cytokines or endotoxin. This inducible transmembrane protein and member of the immunoglobulin gene superfamily was previously reported to contain six immunoglobulinlike domains. Using the polymerase chain reaction, a VCAM-1 cDNA was obtained from mRNA of interleukin-1 (IL-1)-treated cultured human umbilical vein endothelial cells (HUVEC). The cDNA clone contained an additional 276 base-pair (bp) domain, located between domains 3 and 4. This new domain is most homologous to the existing N-terminal domain (domain 1). The internal 276-bp region is encoded by a single exon of the human VCAM-1 gene, indicating that the two forms of mRNA arise by alternative splicing. Both forms of VCAM-1 mRNA were detected by polymerase chain reaction in IL-1-stimulated HUVEC, although the seven-domain form appeared predominant. On the surface of HUVEC only a 110-kd polypeptide, consistent with the seven-immunoglobulinlike domain form of VCAM-1, was detectable by immunoprecipitation. Alternative splicing of the VCAM-1 gene in cytokine-activated endothelium may generate functionally distinct cell-surface adhesion molecules.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-1689216, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-1691264, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-1699207, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-1704191, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-1990440, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-2466335, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-2588007, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-2646860, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-2688898, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-2887295, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-3340213, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-3349522, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-3576199, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/1707234-7063411
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
815-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Alternative splicing of human VCAM-1 in activated vascular endothelium.
pubmed:affiliation
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't