Source:http://linkedlifedata.com/resource/pubmed/id/17070076
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2006-11-13
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| pubmed:abstractText |
Chromatin dynamics that regulate Ifng gene expression are incompletely understood. By using cross-species comparative sequence analyses, we have identified conserved noncoding sequences (CNSs) upstream of the Ifng gene, one of which, located -22 kb from the transcriptional start site, contains clustered consensus binding sequences of transcription factors that function in T cell differentiation. CNS-22 was uniquely associated with histone modifications typical of accessible chromatin in both T helper 1 (Th1) and Th2 cells and demonstrated significant and selective T-bet (T-box transcription factor expressed in T cells, Tbx21)-dependent binding and enhancer activity in Th1 cells. Deletion of CNS-22 in the context of an Ifng reporter transgene ablated T cell receptor-dependent and -independent Ifng expression in Th1 effectors and similarly blocked expression by cytotoxic T lymphocytes and natural killer cells. Thus, a single distal element may be essential for Ifng gene expression by both innate and adaptive immune effector cell lineages.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
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| pubmed:issn |
1074-7613
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
717-29
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17070076-Animals,
pubmed-meshheading:17070076-Base Sequence,
pubmed-meshheading:17070076-Cattle,
pubmed-meshheading:17070076-Cell Differentiation,
pubmed-meshheading:17070076-Chickens,
pubmed-meshheading:17070076-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:17070076-Conserved Sequence,
pubmed-meshheading:17070076-Gene Expression,
pubmed-meshheading:17070076-Gene Expression Regulation,
pubmed-meshheading:17070076-Histones,
pubmed-meshheading:17070076-Interferon-gamma,
pubmed-meshheading:17070076-Killer Cells, Natural,
pubmed-meshheading:17070076-Mice,
pubmed-meshheading:17070076-Mice, Transgenic,
pubmed-meshheading:17070076-Molecular Sequence Data,
pubmed-meshheading:17070076-Opossums,
pubmed-meshheading:17070076-Polymerase Chain Reaction,
pubmed-meshheading:17070076-Rats,
pubmed-meshheading:17070076-Regulatory Elements, Transcriptional,
pubmed-meshheading:17070076-T-Lymphocytes
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
A distal conserved sequence element controls Ifng gene expression by T cells and NK cells.
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| pubmed:affiliation |
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|