Linked Life Data

17066813

Source:http://linkedlifedata.com/resource/pubmed/id/17066813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-10-27
pubmed:abstractText
Our previous studies showed that enhanced cytochrome P450-mediated detoxification is a major mechanism responsible for pyrethroid resistance in a laboratory-selected strain (YGF) of Helicoverpa armigera (Hübner). Two new cytochrome P450 genes, CYP9A12 and CYP9A14 (encoding 531 and 530 amino acid residues, respectively) were isolated from the fat body of the YGF strain of H. armigera. The mRNA expression levels of these two CYP9 P450 genes, together with CYP6B7 and CYP4G8 (previously reported to be overexpressed in pyrethroid-resistant strains), were assessed by real-time quantitative reverse transcription-polymerase chain reaction in the final instars of a field-derived YG strain (with seven-fold resistance to the pyrethroid fenvalerate) and the YGF strain (selected from the YG strain with fenvalerate in the laboratory, with 1,690-fold resistance to fenvalerate). The mRNA expression levels of CYP9A12, CYP9A14, and CYP6B7 in the fat body of the YGF strain increased to 433-, 59-, and 9.3-fold, respectively, compared with the YG strain, whereas no overexpression was revealed for CYP4G8. CYP9A12 and CYP9A14 had 19- and 4.3-fold overexpression in the midgut of the YGF strain compared with the YG strain, respectively, but CYP6B7 and CYP4G8 were not overexpressed. The current study provided evidence that constitutive overexpression of multiple cytochrome P450 genes (CYP9A12, CYP9A14, and CYP6B7) is associated with pyrethroid resistance in H. armigera.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-0493
pubmed:author
pubmed:issnType
Print
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1784-9
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Constitutive overexpression of multiple cytochrome P450 genes associated with pyrethroid resistance in Helicoverpa armigera.
pubmed:affiliation
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't