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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-10-26
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pubmed:abstractText |
Replication of the hepatitis B virus is suppressed by deficiency of the X protein. Although several molecules that block cellular targets of X protein reduce the production of hepatitis B virus progeny, the effect of a specific inhibitor of X protein on viral replication has not been investigated. To block X protein specifically, we adopted an intracellular expression approach using H7 single chain variable fragment (H7scFv), an antibody fragment against X protein. We previously demonstrated that cytoplasmic expression of H7scFv inhibits X protein-induced tumorigenicity and transactivation. In this study, intracellular H7scFv expression inhibits reporter gene transactivation but not viral replication determined by endogenous hepatitis B virus polymerase activity assay and real-time PCR. Our findings imply that intracellular expression of antibody fragment against X protein may not be an alternative therapeutic modality for inhibition of hepatitis B virus replication.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-10049788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-10385671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-10684300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-10998339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-11083647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-11257193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-11536230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-11743208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-11836439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-11961252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-12039955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-15063113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-15078938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-15542625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-15767425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-16273352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-1639069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-1731101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-2034275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-2507484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-2834034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-7588604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-7914435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-8107266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-8764004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-9191947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-9499022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17066517-9572486
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0513-5796
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Intracellular antibody fragment against hepatitis B virus X protein does not inhibit viral replication.
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pubmed:affiliation |
Department of Microbiology, Ajou University School of Medicine, San 5 Wonchon-dong Youngtong-gu, Suwon 443-749, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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