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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-10-26
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pubmed:abstractText |
To investigate pyruvate dehydrogenase (PDH)-E1alpha subunit phosphorylation and whether free fatty acids (FFAs) regulate PDH activity, seven subjects completed two trials: saline (control) and intralipid/heparin (intralipid). Each infusion trial consisted of a 4-h rest followed by a 3-h two-legged knee extensor exercise at moderate intensity. During the 4-h resting period, activity of PDH in the active form (PDHa) did not change in either trial, yet phosphorylation of PDH-E1alpha site 1 (PDH-P1) and site 2 (PDH-P2) was elevated in the intralipid compared with the control trial. PDHa activity increased during exercise similarly in the two trials. After 3 h of exercise, PDHa activity remained elevated in the intralipid trial but returned to resting levels in the control trial. Accordingly, in both trials PDH-P1 and PDH-P2 decreased during exercise, and the decrease was more marked during intralipid infusion. Phosphorylation had returned to resting levels at 3 h of exercise only in the control trial. Thus, an inverse association between PDH-E1alpha phosphorylation and PDHa activity exists. Short-term elevation in plasma FFA at rest increases PDH-E1alpha phosphorylation, but exercise overrules this effect of FFA on PDH-E1alpha phosphorylation leading to even greater dephosphorylation during exercise with intralipid infusion than with saline.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0012-1797
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3020-7
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pubmed:meshHeading |
pubmed-meshheading:17065338-Adult,
pubmed-meshheading:17065338-DNA, Complementary,
pubmed-meshheading:17065338-Enzyme Activation,
pubmed-meshheading:17065338-Exercise,
pubmed-meshheading:17065338-Fat Emulsions, Intravenous,
pubmed-meshheading:17065338-Fatty Acids, Nonesterified,
pubmed-meshheading:17065338-Glycogen,
pubmed-meshheading:17065338-Humans,
pubmed-meshheading:17065338-Infusions, Intravenous,
pubmed-meshheading:17065338-Knee Joint,
pubmed-meshheading:17065338-Mitochondria, Muscle,
pubmed-meshheading:17065338-Muscle, Skeletal,
pubmed-meshheading:17065338-Phosphorylation,
pubmed-meshheading:17065338-Pyruvate Dehydrogenase (Lipoamide),
pubmed-meshheading:17065338-RNA,
pubmed-meshheading:17065338-Rest
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pubmed:year |
2006
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pubmed:articleTitle |
PDH-E1alpha dephosphorylation and activation in human skeletal muscle during exercise: effect of intralipid infusion.
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pubmed:affiliation |
August Krogh Building, Universitetsparken 13, 2100 Copenhagen Ø, Denmark. hpilegaard@aki.ku.dk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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