Linked Life Data

1706380

Source:http://linkedlifedata.com/resource/pubmed/id/1706380

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-4-22
pubmed:abstractText
Although cortical (CD4+CD8+) thymocytes mobilize intracellular calcium poorly when CD3/TCR is ligated, we have found that murine cortical thymocytes can transduce strong biochemical signals in response to ligation of the CD3/Ti TCR complex (CD3/TCR) and that the signals are regulated by CD4 and CD8 interactions with CD3/TCR. Striking increases in intracellular calcium were observed in cortical thymocytes from transgenic mice containing productively rearranged alpha and beta TCR genes, when CD3 or TCR was cross-linked with CD4 or CD8 using heteroconjugated mAb. However, in mature T cells derived from lymph nodes of these mice, identical stimuli elicited calcium responses that were significantly smaller in magnitude. A thymocyte cell line that expresses a low level of the transgenic TCR and has a phenotype characteristic of cortical thymocytes (CD4+CD8+J11d+Thy-1+) was established from a female alpha beta TCR transgenic mouse. Cross-linking of CD4 or CD8 molecules to CD3/TCR induced strong calcium responses in these cells. Responses were weak or absent when CD3 or TCR were aggregated alone. Heteroconjugates of Thy-1xCD3 did not increase the intracellular calcium concentration in transgenic thymocytes or in the thymocyte cell line, although Thy-1 is highly expressed on immature cells. Enhanced tyrosine phosphorylation was observed when CD3 or TCR was cross-linked with CD4 or CD8 on transgenic thymocytes or on the thymocyte cell line, in comparison with aggregation of CD3/TCR alone. Taken together, these data show that CD4 and CD8 molecules allow the weakly expressed CD3/TCR of cortical thymocytes to transduce strong intracellular signals upon receptor ligation. These signals may be involved in selection processes at the CD4+CD8+ stage of differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1759-65
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:1706380-Animals, pubmed-meshheading:1706380-Antigens, CD3, pubmed-meshheading:1706380-Antigens, CD4, pubmed-meshheading:1706380-Antigens, CD8, pubmed-meshheading:1706380-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:1706380-Calcium, pubmed-meshheading:1706380-Cell Differentiation, pubmed-meshheading:1706380-Cell Line, pubmed-meshheading:1706380-Cross-Linking Reagents, pubmed-meshheading:1706380-Female, pubmed-meshheading:1706380-Immune Tolerance, pubmed-meshheading:1706380-Mice, pubmed-meshheading:1706380-Mice, Inbred C57BL, pubmed-meshheading:1706380-Phosphotyrosine, pubmed-meshheading:1706380-Receptors, Antigen, T-Cell, pubmed-meshheading:1706380-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:1706380-Signal Transduction, pubmed-meshheading:1706380-T-Lymphocytes, pubmed-meshheading:1706380-Tyrosine
pubmed:year
1991
pubmed:articleTitle
CD4 and CD8 are positive regulators of T cell receptor signal transduction in early T cell differentiation.
pubmed:affiliation
Oncogen/Bristol-Myers Squibb, Seattle, WA 98121.
pubmed:publicationType
Journal Article