Linked Life Data

1706189

Source:http://linkedlifedata.com/resource/pubmed/id/1706189

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
37
pubmed:dateCreated
1991-4-19
pubmed:abstractText
This project tested the hypothesis that inhaled oxidants could cause lung damage by inactivating the proteinase inhibitors that normally protect the lung from proteolysis. Rat alpha-1-proteinase inhibitor (alpha 1-PI)2 was purified from blood plasma, and antibodies to this inhibitor were prepared. The activity of alpha 1-PI in lung lavage fluids from rats was measured by elastase inhibition, and the immunological concentration of alpha 1-PI was quantified in an enzyme-linked immunoassay. The ratio of the amount of active alpha 1-PI relative to its immunological concentration was examined as a measure of the inhibitor's functional activity. This ratio and the ratio of the immunological concentration of alpha 1-PI to the total protein concentration were determined in lung lavage fluids from rats exposed to air, 10 parts per million (ppm) nitrogen dioxide, and diesel emissions (3.5 mg/m3 particulates) for 12, 18, and 24 months. Only diesel exposures resulted in a statistically significant reduction in the functional activity of alpha 1-PI of 30 percent (p less than 0.05). Similar studies were performed on rats exposed to nitrogen dioxide (0.5 ppm background with peaks of 1.5 ppm) and ozone (0.06 ppm background with peaks of 0.25 ppm) for 12 and 18 months. No statistically significant effects were observed in the functional activity of alpha 1-PI or its immunological concentration. In other protocols, rats were acutely exposed to 0.8 ppm or 1.2 ppm ozone for two, four, or eight hours, and to 0.5 ppm or 0.8 ppm ozone in conjunction with 8 percent carbon dioxide for two or seven hours. Although these acute exposure conditions did not reduce the functional activity of alpha 1-PI, the immunological concentration of alpha 1-PI and the elastase inhibitory activity, relative to other proteins, were significantly increased in relation to the total amount of ozone inhaled. The functional activity of alpha 1-PI also was measured in the bronchoalveolar lavage fluids of human subjects exposed to nitrogen dioxide (0.05 ppm with 2 ppm peaks, or to 1.5 ppm continuously) for three hours and to ozone (0.4 ppm) for two hours during exercise. These exposures did not result in significant changes in the functional activity of alpha 1-PI or its immunological concentration.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1041-5505
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-39
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1706189-Air Pollutants, pubmed-meshheading:1706189-Animals, pubmed-meshheading:1706189-Bronchoalveolar Lavage Fluid, pubmed-meshheading:1706189-Exercise Test, pubmed-meshheading:1706189-Humans, pubmed-meshheading:1706189-Leukocytes, pubmed-meshheading:1706189-Lung, pubmed-meshheading:1706189-Lung Diseases, Obstructive, pubmed-meshheading:1706189-Male, pubmed-meshheading:1706189-Mast Cells, pubmed-meshheading:1706189-Nitrogen Dioxide, pubmed-meshheading:1706189-Oxidants, Photochemical, pubmed-meshheading:1706189-Ozone, pubmed-meshheading:1706189-Pancreatic Elastase, pubmed-meshheading:1706189-Peptide Hydrolases, pubmed-meshheading:1706189-Protease Inhibitors, pubmed-meshheading:1706189-Rats, pubmed-meshheading:1706189-Rats, Inbred F344, pubmed-meshheading:1706189-Vehicle Emissions, pubmed-meshheading:1706189-alpha 1-Antitrypsin
pubmed:year
1990
pubmed:articleTitle
Oxidant effects on rat and human lung proteinase inhibitors.
pubmed:affiliation
Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0002.
pubmed:publicationType
Journal Article