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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
43
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pubmed:dateCreated |
2006-10-25
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pubmed:abstractText |
Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-11195491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-14744438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-15496526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-15684409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-15782179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16043497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16141061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16314309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16503132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16503596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16540696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16643008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17061884-16732647
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-7863
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14044-6
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions.
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pubmed:affiliation |
Department of Chemistry, University of California-Irvine, Irvine, CA 92697, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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