Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-4-8
pubmed:databankReference
pubmed:abstractText
The neuron-glia cell adhesion molecule (Ng-CAM) mediates both neuron-neuron and neuron-glia adhesion; it is detected on SDS-PAGE as a predominant 135-kD glycoprotein, with minor components of 80, 190, and 210 kD. We have isolated cDNA clones encoding the entire sequence of chicken Ng-CAM. The predicted extracellular region includes six immunoglobulin-like domains followed by five fibronectin-type III repeats, structural features that are characteristic of several neural CAMs of the N-CAM superfamily. The amino acid sequence of chicken Ng-CAM is most similar to that of mouse L1 but the overall identity is only 40% and Ng-CAM contains a short fibronectin-like segment with an RGD sequence that has no counterpart in L1. These findings suggest that Ng-CAM and L1 may not be equivalent molecules in chicken and mouse. The amino-terminal sequences of the 210-, 190-, and 135-kD components of Ng-CAM are all the same as the predicted amino terminus of the molecule, whereas the 80-kD component begins within the third fibronectin repeat. The cDNA sequence is continuous across the junction between the 135- and 80-kD components, and a single 170-kD Ng-CAM polypeptide was isolated from tunicamycin-treated cells. In addition, all cDNA probes hybridized on Northern blots to a 6-kb RNA, and most hybridized to single bands on Southern blots. These results indicate that the Ng-CAM components are derived from a single polypeptide encoded by a single gene, and that the 135- and 80-kD components are generated from the 210/190-kD species by proteolytic cleavage. The 135-kD component contains most of the extracellular region including all of the immunoglobulin-like domains. It has no transmembrane segment, but it is tightly associated with the membrane. The 80-kD component contains two and a half type III repeats plus the RGD-containing segment, as well as the single transmembrane and cytoplasmic domains. These structural features of Ng-CAM provide a framework for understanding its multiple functions in neuron-neuron interactions, neurite fasciculation, and neuron-glia interactions.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3467341, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3472238, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3458261, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3684567, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3677174, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3863135, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-4092678, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-4033770, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3991809, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3981244, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2317872, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-1693086, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2295683, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6198242, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6172996, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6814825, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6206400, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6315951, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6325925, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6329026, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6219389, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6368220, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6383201, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6364137, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6364040, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6356359, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6344077, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6725397, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2466966, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2443800, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3611052, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3595563, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3576199, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3559708, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3805123, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3780668, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3760944, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3881458, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3856258, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6655495, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6621692, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-7023366, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-6959151, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-7108955, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-218819, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-320200, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-4598444, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-5650387, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2253323, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2474555, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3412448, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3416203, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3162770, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3049624, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3714490, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2448316, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2992362, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2443794, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2805067, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3187519, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3028640, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2992939, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2956925, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3141062, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3048970, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-3289571, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2438699, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2445560, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2432614, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2435742, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2420947, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2426400, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2415352, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2415980, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2578885, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2509484, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2677246, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2627374, http://linkedlifedata.com/resource/pubmed/commentcorrection/1705558-2624745
More...