Source:http://linkedlifedata.com/resource/pubmed/id/17055347
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2007-1-29
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| pubmed:abstractText |
A liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) assay for the determination of bencycloquidium bromide (BCQB) in rat plasma was firstly developed and validated. After addition of 1-ethyl-bencycloquidium bromide as an internal standard (I.S.), the plasma samples were deproteinized with methanol and the supernatant was assayed by LC-ESI-MS. Chromatographic separation was achieved with a Hanbon Lichrospher 5-C18 column. The mobile phase consisted of methanol-40 mM ammonium acetate buffer-formic acid (75:25:0.25, v/v/v) and delivered at the flow rate of 1.0 ml/min. LC-ESI-MS was carried out on a single quadrupole mass spectrometer using electrospray ionization (ESI) and positive selected-ion monitoring (SIM). Target ions were monitored at [M](+)m/z 330.2 for BCQB and [M] (+)m/z 344.2 for I.S. Calibration curve was linear over the range of 3-1500 ng/ml. The lower limit of quantification (LLOQ) was 3.0 ng/ml. The intra- and inter-run relative standard deviations (R.S.D.%) of the assay were less than 7.1 and 12.3%, respectively. The accuracy determined at the concentrations of 3.0, 100.0, 500.0 and 1500 ng/ml for BCQB were within +/-15.0%. The established method has been applied successfully to study the pharmacokinetics of BCQB in rats after intranasal administration.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1570-0232
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
846
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
209-14
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| pubmed:meshHeading |
pubmed-meshheading:17055347-Animals,
pubmed-meshheading:17055347-Bicyclo Compounds, Heterocyclic,
pubmed-meshheading:17055347-Calibration,
pubmed-meshheading:17055347-Cholinergic Antagonists,
pubmed-meshheading:17055347-Chromatography, Liquid,
pubmed-meshheading:17055347-Rats,
pubmed-meshheading:17055347-Reference Standards,
pubmed-meshheading:17055347-Reproducibility of Results,
pubmed-meshheading:17055347-Sensitivity and Specificity,
pubmed-meshheading:17055347-Spectrometry, Mass, Electrospray Ionization
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| pubmed:year |
2007
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| pubmed:articleTitle |
Determination of bencycloquidium bromide in rat plasma by liquid chromatography-electrospray ionization-mass spectrometry.
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| pubmed:affiliation |
Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
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| pubmed:publicationType |
Journal Article
|