Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-1-8
pubmed:abstractText
Resolvin D1 (RvD1) and protectin D1 (Neuroprotectin D1, PD1/NPD1) are newly identified anti-inflammatory lipid mediators biosynthesized from docosahexaenoic acid (DHA). In this report, the spectra-structure correlations and fragmentation mechanisms were studied using electrospray low-energy collision-induced dissociation tandem mass spectrometry (MS/MS) for biogenic RvD1 and PD1, as well as mono-hydroxy-DHA and related hydroperoxy-DHA. The loss of H2O and CO2 in the spectra indicates the number of functional group(s). Chain-cut ions are the signature of the positions and numbers of functional groups and double bonds. The observed chain-cut ion is equivalent to a hypothetical homolytic-segment (cc, cm, mc, or mm) with addition or extraction of up to 2 protons (H). The alpha-cleavage ions are equivalent to: [cc + H], with H from the hydroxyl through a beta-ene or gamma-ene rearrangement; [cm - 2H], with 2H from hydroxyls of PD1 through a gamma-ene rearrangement, or 1H from the hydroxyl and the other H from the alpha-carbon of mono-HDHA through an alpha-H-beta-ene rearrangement; [mc - H], with H from hydroxyl through a beta-ene or gamma-ene rearrangement, or from the alpha-carbon through an alpha-H-beta-ene rearrangement; or [mm] through charge-direct fragmentations. The beta-ene or gamma-ene facilitates the H shift to gamma position and alpha-cleavage. Deuterium labeling confirmed the assignment of MS/MS ions and the fragmentation mechanisms. Based on the MS/MS spectra and fragmentation mechanisms, we identified RvD1, PD1, and mono-hydroxy-DHA products in human neutrophils and blood, trout head-kidney, and stroke-injury murine brain-tissue.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-10465168, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-10976887, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-11199379, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-11712255, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-12391014, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-12590139, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-12820273, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-12923200, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-12923235, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-14515314, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-14762183, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-15152078, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-15282772, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-15532045, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-15722568, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-15961057, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-16303609, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-16424216, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-16443367, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-2147773, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-2541468, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-6234372, http://linkedlifedata.com/resource/pubmed/commentcorrection/17055291-9808710
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-0305
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
128-44
pubmed:dateRevised
2011-4-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Resolvin D1, protectin D1, and related docosahexaenoic acid-derived products: Analysis via electrospray/low energy tandem mass spectrometry based on spectra and fragmentation mechanisms.
pubmed:affiliation
Analytical Core, Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural