Source:http://linkedlifedata.com/resource/pubmed/id/17054986
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2006-11-24
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| pubmed:abstractText |
In our search for new partners of the HIV-1 envelope glycoprotein (Env), we found that the cytoplasmic domain of the TMgp41 (TMgp41 CD) subunit of HIV-1 Env interacted with Luman, a transcription factor of the CREB/ATF family. Luman is anchored in the endoplasmic reticulum membrane and subjected to activation by regulated intramembrane proteolysis (RIP). The RIP process permits the release of the activated amino-terminal fragment of Luman into the cytoplasm, and its import into the nucleus. Here, we demonstrate that interaction between the TMgp41 CD and Luman requires a region encompassing the b-Zip and TM domains of Luman and decreases the stability of this factor. Moreover, we found that overexpression of a constitutively active form of Luman in cells transfected with HXB2R HIV-1 provirus decreased the intracellular expression of Gag and Env and led to a decrease in virion release. This negative effect of activated Luman on HIV-1 production was correlated to the inhibition of Tat transactivation of the HIV-1 LTR, which might be related to an interaction of activated Luman with Tat. Altogether, these results show that Luman acts as a partner of two major HIV-1 proteins: the TMgp41 Env subunit and Tat. The interaction between the TMgp41 subunit of Env and Luman affects the stability of the full-length Luman protein, the precursor of the activated, nuclear form of Luman, which acts negatively on Tat-mediated HIV-1 transactivation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CREB3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp41,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0022-2836
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
364
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1034-47
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17054986-Cells, Cultured,
pubmed-meshheading:17054986-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:17054986-Cytoplasm,
pubmed-meshheading:17054986-Endoplasmic Reticulum,
pubmed-meshheading:17054986-Gene Expression Regulation, Viral,
pubmed-meshheading:17054986-Gene Products, gag,
pubmed-meshheading:17054986-Gene Products, tat,
pubmed-meshheading:17054986-Genes, gag,
pubmed-meshheading:17054986-HIV Envelope Protein gp41,
pubmed-meshheading:17054986-HIV Long Terminal Repeat,
pubmed-meshheading:17054986-HIV-1,
pubmed-meshheading:17054986-Humans,
pubmed-meshheading:17054986-Immunoprecipitation,
pubmed-meshheading:17054986-Proviruses,
pubmed-meshheading:17054986-Saccharomyces cerevisiae,
pubmed-meshheading:17054986-Transcription, Genetic,
pubmed-meshheading:17054986-Transcriptional Activation,
pubmed-meshheading:17054986-Two-Hybrid System Techniques,
pubmed-meshheading:17054986-Virus Replication,
pubmed-meshheading:17054986-tat Gene Products, Human Immunodeficiency Virus
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| pubmed:year |
2006
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| pubmed:articleTitle |
Luman, a new partner of HIV-1 TMgp41, interferes with Tat-mediated transcription of the HIV-1 LTR.
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| pubmed:affiliation |
Institut Cochin, Département Maladies Infectieuses, Paris F-75014, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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