Source:http://linkedlifedata.com/resource/pubmed/id/17050058
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2007-2-5
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pubmed:abstractText |
Baicalein is known as a 12-lipoxygenase (12-LOX) inhibitor. The 12-LOX is found to be involved in the progression of human cancers and the inhibitor of 12-LOX offers a target for the prevention cancer. We demonstrated the inhibitory effect of baicalein on the gene and protein expression of 12-LOX in H460 human lung nonsmall carcinoma cell line. Treatment of baicalein inhibited the growth of H460 cells in a dose-dependent manner. Following 24h exposure to 50muM baicalein, cell cycle analysis revealed an increase in the cell population in S-phase. During the S-phase arrest, baicalein decreased the protein levels of cdk1 and cyclin B1, which are the regulating proteins of S-phase transition to G2/M-phase, in this study. Furthermore, baicalein induced the most of H460 cell apoptosis after treatment for 48h. H460 cells formed vesicles and apoptotic body, and then floated after treatment with baicalein. Baicalein-induced H460 cell apoptosis was confirmed by DNA condensation and fragmentation. Baicalein-induced apoptosis were also accompanied by decreasing in Bcl-2 and proform of caspase-3 and increasing p53 and Bax protein levels. Pretreatment with a specific caspase-3 inhibitor, Ac-DEVD-CHO, partially reduced baicalein-induced cell death, indicating baicalein induces apoptosis is partially dependent on caspase-3 pathway in H460 cells. These data suggest that baicalein, a 12-LOX inhibitor, inhibits the proliferation of H460 cells via S-phase arrest and induces apoptosis in association with the regulation of molecules in the cell cycle and apoptosis-related proteins.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Flavanones,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/baicalein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0278-6915
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
403-11
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:17050058-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:17050058-Apoptosis,
pubmed-meshheading:17050058-Blotting, Western,
pubmed-meshheading:17050058-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:17050058-Cell Line, Tumor,
pubmed-meshheading:17050058-DNA Primers,
pubmed-meshheading:17050058-Flavanones,
pubmed-meshheading:17050058-Flow Cytometry,
pubmed-meshheading:17050058-Humans,
pubmed-meshheading:17050058-Lipoxygenase Inhibitors,
pubmed-meshheading:17050058-Lung Neoplasms,
pubmed-meshheading:17050058-Phytotherapy,
pubmed-meshheading:17050058-RNA,
pubmed-meshheading:17050058-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17050058-Scutellaria baicalensis
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pubmed:year |
2007
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pubmed:articleTitle |
Inhibition of 12-lipoxygenase during baicalein-induced human lung nonsmall carcinoma H460 cell apoptosis.
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pubmed:affiliation |
Department of Radiation Oncology, Chi Mei Hospital, Liouying, Tainan, Taiwan.
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pubmed:publicationType |
Journal Article
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