Linked Life Data

17049431

Source:http://linkedlifedata.com/resource/pubmed/id/17049431

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-11-1
pubmed:abstractText
Edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. Resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. Here we show that intracellular replication of E. tarda in murine macrophages is dependent on the type III secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic NF-kappaB target genes. The wild-type strain replicates within the phagosomal membrane of macrophages; whereas the type III mutant does not. Microarray analysis shows the mRNA expression level of NF-kappaB target genes (e.g. pro-inflammatory cytokines and anti-apoptotic genes) in macrophages infected with the wild-type strain were up-regulated compared to macrophages infected with the type III mutant. Up-regulation of Bcl2a1a, Bcl2a1b, cIAP-2, and TRAF1 genes induced expression of anti-apoptotic proteins to protect macrophages from apoptosis induced by staurosporine. Further, this protection was inhibited by adding kamebakaurin, an inhibitor of NF-kappaB activation and was confirmed using an NF-kappaB reporter gene assay. Up-regulation of anti-apoptotic NF-kappaB target genes is responsible for the anti-apoptotic activity of E. tarda and is required for intracellular replication in murine macrophages.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0882-4010
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
226-40
pubmed:meshHeading
pubmed-meshheading:17049431-Animals, pubmed-meshheading:17049431-Apoptosis, pubmed-meshheading:17049431-Blotting, Western, pubmed-meshheading:17049431-Cell Line, pubmed-meshheading:17049431-DNA, Bacterial, pubmed-meshheading:17049431-Diterpenes, pubmed-meshheading:17049431-Edwardsiella tarda, pubmed-meshheading:17049431-Enterobacteriaceae Infections, pubmed-meshheading:17049431-Gene Expression Regulation, Bacterial, pubmed-meshheading:17049431-Macrophages, pubmed-meshheading:17049431-Mice, pubmed-meshheading:17049431-Microscopy, Electron, Transmission, pubmed-meshheading:17049431-Mutagenesis, Insertional, pubmed-meshheading:17049431-NF-kappa B, pubmed-meshheading:17049431-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17049431-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:17049431-Staurosporine, pubmed-meshheading:17049431-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-kappaB target genes protecting the macrophage from staurosporine-induced apoptosis.
pubmed:affiliation
Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4, Kagamiyama, Higashi-Hiroshima, 739-8528, Japan. okudaj@hiroshima-u.ac.jp <okudaj@hiroshima-u.ac.jp>
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't