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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
43
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pubmed:dateCreated |
2006-10-25
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pubmed:abstractText |
Adrenomedullin (AM) belongs to the calcitonin gene-related peptide (CGRP) family and is a well known potent vasodilator. We show here that AM is a powerful pain-inducing neuropeptide. AM-like immunoreactivity is widely distributed in both CGRP-containing and lectin IB4-binding nociceptors in dorsal root ganglion and axon terminals in the superficial dorsal horn of the rat spinal cord. Specific binding sites for the radioligand, [(125)I]AM13-52 as well as immunoreactivity for receptor markers such as the calcitonin receptor-like receptor and three receptor-activity-modifying proteins are localized in the superficial dorsal horn, demonstrating the existence of AM/CGRP receptors in this region. Intrathecal injection of rat AM1-50, dose- and time-dependently, induced long-lasting heat hyperalgesia and increased the phosphorylation of Akt and GSK3beta in the dorsal horn. Pre- and posttreatments with the AM receptor antagonist AM22-52 and PI3 kinase inhibitors (LY294002 and Wortmannin) significantly blocked or reversed AM-induced heat hyperalgesia. Pre- and posttreatments with AM22-52 and Wortmannin also significantly blocked or reversed intraplantar capsaicin-induced heat hyperalgesia. Taken together, our results demonstrate that AM acts as a pain-inducing peptide in the dorsal horn. By activating specific receptors (likely AM2) and the PI3K/Akt/GSK3beta signaling pathway, AM could play a significant role in long-lasting heat hypersensitivity and inflammatory heat hyperalgesia.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-10103088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-11121574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-11440979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-11602001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-12037140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-12529937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-12895509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-14511336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-14691041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-15165714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-15269340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-15385613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-15464589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-15670621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-16082677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-16118273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-1637079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-2452992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-6209366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-8622957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-9176019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-9349813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-9353797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-9581756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17043245-9620797
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenomedullin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin Gene-Related Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Capsaicin,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Activity-Modifying Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenomedullin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Peptide
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16027-32
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:17043245-Adrenomedullin,
pubmed-meshheading:17043245-Animals,
pubmed-meshheading:17043245-Behavior, Animal,
pubmed-meshheading:17043245-Binding Sites,
pubmed-meshheading:17043245-Calcitonin Gene-Related Peptide,
pubmed-meshheading:17043245-Capsaicin,
pubmed-meshheading:17043245-Enzyme Activation,
pubmed-meshheading:17043245-Ganglia, Spinal,
pubmed-meshheading:17043245-Inflammation,
pubmed-meshheading:17043245-Injections, Spinal,
pubmed-meshheading:17043245-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:17043245-Male,
pubmed-meshheading:17043245-Membrane Proteins,
pubmed-meshheading:17043245-Nociceptors,
pubmed-meshheading:17043245-Pain,
pubmed-meshheading:17043245-Phosphotransferases,
pubmed-meshheading:17043245-Rats,
pubmed-meshheading:17043245-Rats, Sprague-Dawley,
pubmed-meshheading:17043245-Receptor Activity-Modifying Proteins,
pubmed-meshheading:17043245-Receptors, Adrenomedullin,
pubmed-meshheading:17043245-Receptors, Peptide,
pubmed-meshheading:17043245-Signal Transduction
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pubmed:year |
2006
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pubmed:articleTitle |
A role for adrenomedullin as a pain-related peptide in the rat.
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pubmed:affiliation |
Douglas Hospital Research Center, and Department of Psychiatry, McGill University, Montreal, QC, Canada H4H 1R3.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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