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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0009017,
umls-concept:C0017262,
umls-concept:C0085983,
umls-concept:C0185117,
umls-concept:C1337092,
umls-concept:C1452296,
umls-concept:C1522558,
umls-concept:C1705079,
umls-concept:C1882867,
umls-concept:C2911684
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pubmed:issue |
2
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|
pubmed:dateCreated |
1991-3-8
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pubmed:databankReference |
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pubmed:abstractText |
Rat monocyte chemoattractant protein-1 (MCP-1) cDNA was cloned. A cDNA library was constructed from mRNA extracted from Con A-stimulated rat spleen cells. After 2 rounds of screening with a radiolabeled oligonucleotide probe and DNA sequencing from plasmid DNAs, one clone which encoded for MCP-1 was obtained. The cDNA clone comprises 665 base pairs with an open reading frame which encodes for 148 amino acid protein. Spleen cells expressed a significant amount of MCP-1 mRNA without stimulus. But higher expression was observed when cells were stimulated with Con A. MCP-1 mRNA was also expressed in tumor cell lines, although the amount of expressed MCP-1 mRNA was different among cell lines.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
504-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1704226-Amino Acid Sequence,
pubmed-meshheading:1704226-Animals,
pubmed-meshheading:1704226-Base Sequence,
pubmed-meshheading:1704226-Blotting, Northern,
pubmed-meshheading:1704226-Blotting, Southern,
pubmed-meshheading:1704226-Chemokine CCL2,
pubmed-meshheading:1704226-Chemotactic Factors,
pubmed-meshheading:1704226-Chemotaxis, Leukocyte,
pubmed-meshheading:1704226-Cloning, Molecular,
pubmed-meshheading:1704226-Concanavalin A,
pubmed-meshheading:1704226-DNA,
pubmed-meshheading:1704226-Gene Expression,
pubmed-meshheading:1704226-Gene Library,
pubmed-meshheading:1704226-Humans,
pubmed-meshheading:1704226-Molecular Sequence Data,
pubmed-meshheading:1704226-Poly A,
pubmed-meshheading:1704226-RNA,
pubmed-meshheading:1704226-RNA, Messenger,
pubmed-meshheading:1704226-Rats,
pubmed-meshheading:1704226-Rats, Inbred F344,
pubmed-meshheading:1704226-Recombinant Proteins,
pubmed-meshheading:1704226-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1704226-Spleen
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pubmed:year |
1991
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pubmed:articleTitle |
Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines.
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pubmed:affiliation |
Immunopathology Section, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.
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pubmed:publicationType |
Journal Article,
Comparative Study
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