Linked Life Data

17040906

Source:http://linkedlifedata.com/resource/pubmed/id/17040906

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
49
pubmed:dateCreated
2006-12-4
pubmed:abstractText
Dicumarol is a naturally occurring anticoagulant derived from coumarin that induces cytotoxicity and oxidative stress in human pancreatic cancer cells (Cullen, J. J., Hinkhouse, M. M., Grady, M., Gaut, A. W., Liu, J., Zhang, Y., Weydert, C. J. D., Domann, F. E., and Oberley, L. W. (2003) Cancer Res. 63, 5513-5520). Although dicumarol has been used as an inhibitor of the two-electron reductase NAD(P)H:quinone oxidoreductase (NQO1), dicumarol is also thought to affect quinone-mediated electron transfer reactions in the mitochondria, leading to the production of superoxide (O2*-) and hydrogen peroxide (H(2)O(2)). We hypothesized that mitochondrial production of reactive oxygen species mediates the increased susceptibility of pancreatic cancer cells to dicumarol-induced metabolic oxidative stress. Dicumarol decreased clonogenic survival equally in both MDA-MB-468 NQO1(-) and MDA-MB-468 NQO1+ breast cancer cells. Dicumarol decreased clonogenic survival in the transformed fibroblast cell line IMRSV-90 compared with the IMR-90 cell line. Dicumarol, with the addition of mitochondrial electron transport chain blockers, decreased clonogenic cell survival in human pancreatic cancer cells and increased superoxide levels. Dicumarol with the mitochondrial electron transport chain blocker antimycin A decreased clonogenic survival and increased superoxide levels in cells with functional mitochondria but had little effect on cancer cells without functional mitochondria. Overexpression of manganese superoxide dismutase and mitochondrial-targeted catalase with adenoviral vectors reversed the dicumarol-induced cytotoxicity and reversed fluorescence of the oxidation-sensitive probe. We conclude mitochondrial production of reactive oxygen species mediates the increased susceptibility of cancer cells to dicumarol-induced cytotoxicity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37416-26
pubmed:dateRevised
2010-4-29
pubmed:meshHeading
pubmed-meshheading:17040906-Antineoplastic Agents, pubmed-meshheading:17040906-Breast Neoplasms, pubmed-meshheading:17040906-Catalase, pubmed-meshheading:17040906-Cell Line, pubmed-meshheading:17040906-Cell Line, Transformed, pubmed-meshheading:17040906-Cell Line, Tumor, pubmed-meshheading:17040906-Cell Survival, pubmed-meshheading:17040906-Dicumarol, pubmed-meshheading:17040906-Electron Transport, pubmed-meshheading:17040906-Female, pubmed-meshheading:17040906-Humans, pubmed-meshheading:17040906-Hydrogen Peroxide, pubmed-meshheading:17040906-Kinetics, pubmed-meshheading:17040906-Mitochondria, pubmed-meshheading:17040906-NAD(P)H Dehydrogenase (Quinone), pubmed-meshheading:17040906-Oxidative Stress, pubmed-meshheading:17040906-Pancreatic Neoplasms, pubmed-meshheading:17040906-Reactive Oxygen Species, pubmed-meshheading:17040906-Recombinant Proteins, pubmed-meshheading:17040906-Superoxide Dismutase, pubmed-meshheading:17040906-Tumor Stem Cell Assay, pubmed-meshheading:17040906-Uncoupling Agents
pubmed:year
2006
pubmed:articleTitle
Mitochondrial production of reactive oxygen species mediate dicumarol-induced cytotoxicity in cancer cells.
pubmed:affiliation
Department of Surgery and University of Iowa College of Medicine, Holden Comprehensive Cancer Center, and Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural