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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-3-4
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pubmed:abstractText |
The gene coding for starch phosphorylase (EC 2.4.1.1) was isolated from a potato genomic library constructed in lambda EMBL3. It is an unusually long plant gene (16.4 kb) which encodes a preprotein of 966 amino acids. The phosphorylase coding sequence is interrupted by 14 introns whose positions do not match those of the introns in the human glycogen phosphorylase gene. A 78 amino acid central peptide unique to plant plastidial phosphorylases is hypothesized to have arisen through the mis-splicing of an intron-exon junction site in an ancestral gene. The fifth intron of the phosphorylase is very large (approximately 7 kb) and contains a copia-like transposable element inserted in the opposite orientation to that of the phosphorylase gene. This element has been named Tst1; it is bordered on the 5' and 3' sides by long terminal repeats of 285 and 283 bp respectively, which define an internal domain of 4492 bp. Tst1 contains 4 open reading frames (ORFs) that encode protein domains for a reverse transcriptase, an integrase, an RNA-binding site and a protease. Transcription of the phosphorylase gene appears to proceed unimpaired through the copia element.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
224
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1703627-Amino Acid Sequence,
pubmed-meshheading:1703627-Base Sequence,
pubmed-meshheading:1703627-Binding Sites,
pubmed-meshheading:1703627-Chloroplasts,
pubmed-meshheading:1703627-DNA Nucleotidyltransferases,
pubmed-meshheading:1703627-DNA Transposable Elements,
pubmed-meshheading:1703627-Endopeptidases,
pubmed-meshheading:1703627-Genes, Plant,
pubmed-meshheading:1703627-Integrases,
pubmed-meshheading:1703627-Introns,
pubmed-meshheading:1703627-Molecular Sequence Data,
pubmed-meshheading:1703627-Open Reading Frames,
pubmed-meshheading:1703627-Phosphorylases,
pubmed-meshheading:1703627-RNA,
pubmed-meshheading:1703627-RNA-Directed DNA Polymerase,
pubmed-meshheading:1703627-Restriction Mapping,
pubmed-meshheading:1703627-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1703627-Solanum tuberosum,
pubmed-meshheading:1703627-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Occurrence of a copia-like transposable element in one of the introns of the potato starch phosphorylase gene.
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pubmed:affiliation |
Département de Biochimie, Université de Montréal, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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