|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1991-3-7
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The inhibitory glycine receptor (GlyR) in mammalian spinal cord displays pharmacological and molecular heterogeneity of its strychnine binding alpha subunit. Here, cDNAs were isolated which encode a variant (alpha ins 1) of the rat GlyR alpha 1 subunit that contains eight additional amino acids in its putative cytoplasmic domain. Analysis of the corresponding genomic sequence showed that alpha ins 1 transcripts result from alternative splice acceptor site selection. S1 nuclease protection experiments, Northern blot analysis, and RNA amplification by polymerase chain reaction revealed alpha 1 and alpha ins 1 mRNA in postnatal spinal cord, but not in other brain regions. Expression of synthetic alpha ins 1 RNA in Xenopus oocytes generated glycine-gated strychnine-sensitive chloride channels. These data indicate that alternative splicing contributes to GlyR alpha subunit heterogeneity in the mammalian central nervous system.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotransmitter,
http://linkedlifedata.com/resource/pubmed/chemical/Strychnine
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
266
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2048-53
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1703526-Amino Acid Sequence,
pubmed-meshheading:1703526-Animals,
pubmed-meshheading:1703526-Base Sequence,
pubmed-meshheading:1703526-Blotting, Northern,
pubmed-meshheading:1703526-Brain,
pubmed-meshheading:1703526-Chloride Channels,
pubmed-meshheading:1703526-Chlorides,
pubmed-meshheading:1703526-Ion Channels,
pubmed-meshheading:1703526-Membrane Proteins,
pubmed-meshheading:1703526-Molecular Sequence Data,
pubmed-meshheading:1703526-Polymerase Chain Reaction,
pubmed-meshheading:1703526-RNA, Messenger,
pubmed-meshheading:1703526-RNA Splicing,
pubmed-meshheading:1703526-Rats,
pubmed-meshheading:1703526-Receptors, Glycine,
pubmed-meshheading:1703526-Receptors, Neurotransmitter,
pubmed-meshheading:1703526-Restriction Mapping,
pubmed-meshheading:1703526-Spinal Cord,
pubmed-meshheading:1703526-Strychnine,
pubmed-meshheading:1703526-Transcription, Genetic,
pubmed-meshheading:1703526-Xenopus
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Alternative splicing generates two variants of the alpha 1 subunit of the inhibitory glycine receptor.
|
|
pubmed:affiliation |
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
