Linked Life Data

1702721

Source:http://linkedlifedata.com/resource/pubmed/id/1702721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-2-20
pubmed:abstractText
The contribution of oligosaccharides to the biochemical composition and antigen heterogeneity of the phosphotyrosine phosphatase CD45 glycoproteins has been studied on the K-562 erythroleukemic cell line. Treatment of immunoprecipitated CD45 glycoproteins with distinct exo- and endoglycosidases revealed the presence of highly sialylated O- and N-linked complex carbohydrates in the composition of mature CD45 glycoproteins. Incubation of K-562 cells with the N-glycosylation inhibitor tunicamycin blocked carbohydrate processing during biosynthesis of CD45 proteins, generating unglycosylated polypeptides similar in size to those resulting from digestion of CD45 proteins with a mixture of both N- and O-glycanases. Epitopes defining the T cell maturation related CD45R0 and CD45RB antigen specificities were present on the mature 180- and 190-kDa K-562 CD45 proteins, respectively. However, the CD45R0 and CD45RB epitopes were not detected on the high mannose biosynthetic CD45 precursors. Furthermore, treatment of CD45 proteins with O-glycanase or neuraminidase resulted in the loss of both CD45R0 and CD45RB epitopes, although reactivity of the anti-CD45R0 and anti-CD45RB mAb was not affected by mAb preincubation with either sialic acids or sialyllactose in solution. From these results we conclude that the blockade of early steps of N-glycosylation during carbohydrate processing resulted in the inhibition of subsequent incorporation of O-linked sugars on CD45 polypeptides, thus preventing the late acquisition of the CD45R0 and CD45RB determinants on the 180- and 190-kDa CD45 polypeptides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2667-71
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Glycosylation of CD45: carbohydrate composition and its role in acquisition of CD45R0 and CD45RB T cell maturation-related antigen specificities during biosynthesis.
pubmed:affiliation
Servicio de Immunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't