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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0026845,
umls-concept:C0036849,
umls-concept:C0220825,
umls-concept:C0224306,
umls-concept:C0599161,
umls-concept:C1135183,
umls-concept:C1418779,
umls-concept:C1442518,
umls-concept:C1511726,
umls-concept:C1514811,
umls-concept:C1527148,
umls-concept:C1552652,
umls-concept:C1552685,
umls-concept:C1561577,
umls-concept:C1705195,
umls-concept:C1706462,
umls-concept:C1882115
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pubmed:dateCreated |
2006-10-16
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pubmed:abstractText |
An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be drawn. This can be done by using one or multiple, validated reference genes, depending on the desired accuracy of the results. In the pig however, very little information is available on the expression stability of reference genes. The aim of this study was therefore to develop a new set of reference genes which can be used for normalization of mRNA expression data of genes expressed in porcine backfat and longissimus dorsi muscle, both representing an economically important part of a pig's carcass. Because of its multiple functions in fat metabolism and muscle fibre type composition, peroxisome proliferative activated receptor gamma coactivator 1alpha (PPARGC1A) is a very interesting candidate gene for meat quality, and was an ideal gene to evaluate our developed set of reference genes for normalization of mRNA expression data of both tissue types.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-10022859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-10412986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-10580157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-10617337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-10834578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-10913342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-11013345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-11086195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-11274399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-11557972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-11793024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-11875072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-12181572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-12565902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-12824337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-12852853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-14570700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-14740362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-15885146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16130456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16130483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16143825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16254077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16274476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16276098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-16553873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-8908518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-9529258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026777-9582194
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1472-6750
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17026777-Adipose Tissue,
pubmed-meshheading:17026777-Animals,
pubmed-meshheading:17026777-Gene Expression,
pubmed-meshheading:17026777-Meat,
pubmed-meshheading:17026777-Muscle, Skeletal,
pubmed-meshheading:17026777-RNA, Messenger,
pubmed-meshheading:17026777-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17026777-Swine,
pubmed-meshheading:17026777-Transcription Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A.
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pubmed:affiliation |
Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium. tim.erkens@ugent.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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