Linked Life Data

17026720

Source:http://linkedlifedata.com/resource/pubmed/id/17026720

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-12-21
pubmed:abstractText
Human papillomaviruses (HPVs) are obligate epithelial pathogens and typically cause localized mucosal infections. We therefore hypothesized that T-cell responses to HPV antigens would be greater at sites of pathology than in the blood. Focusing on HPV-16 because of its association with cervical cancer, the magnitude of HPV-specific T-cell responses at the cervix was compared with those in the peripheral blood by intracellular cytokine staining following direct ex vivo stimulation with both virus-like particles assembled from the major capsid protein L1, and the major HPV oncoprotein, E7. We show that both CD4(+) and CD8(+) T cells from the cervix responded to the HPV-16 antigens and that interferon-gamma (IFN-gamma) production was HPV type-specific. Comparing HPV-specific T-cell IFN-gamma responses at the cervix with those in the blood, we found that while CD4(+) and CD8(+) T-cell responses to L1 were significantly correlated between compartments (P = 0.02 and P = 0.05, respectively), IFN-gamma responses in both T-cell subsets were significantly greater in magnitude at the cervix than in peripheral blood (P = 0.02 and P = 0.003, respectively). In contrast, both CD4(+) and CD8(+) T-cell IFN-gamma responses to E7 were of similar magnitude in both compartments and CD8(+) responses were significantly correlated between these distinct immunological compartments (P = 0.04). We therefore show that inflammatory T-cell responses against L1 (but not E7) demonstrate clear compartmental bias and the magnitude of these responses do reflect local viral replication but that correlation of HPV-specific responses between compartments indicates their linkage.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-10073701, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-10223336, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-10354552, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-10716964, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-10863633, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-11312659, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-11600177, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-11902586, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-11992584, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-12445658, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-12477834, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-12571259, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-12938202, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-14704767, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-15207617, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-15374995, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-15767429, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-15994816, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-15994817, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-7706782, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-9021543, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-9374383, http://linkedlifedata.com/resource/pubmed/commentcorrection/17026720-9459645
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0019-2805
pubmed:author
pubmed:issnType
Print
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
507-14
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:17026720-Adult, pubmed-meshheading:17026720-Antibodies, Viral, pubmed-meshheading:17026720-Antigens, Viral, pubmed-meshheading:17026720-Cervical Intraepithelial Neoplasia, pubmed-meshheading:17026720-Cervix Uteri, pubmed-meshheading:17026720-Cohort Studies, pubmed-meshheading:17026720-Female, pubmed-meshheading:17026720-Human papillomavirus 16, pubmed-meshheading:17026720-Humans, pubmed-meshheading:17026720-Immunity, Cellular, pubmed-meshheading:17026720-Immunoglobulin G, pubmed-meshheading:17026720-Interferon-gamma, pubmed-meshheading:17026720-Leukocyte L1 Antigen Complex, pubmed-meshheading:17026720-Middle Aged, pubmed-meshheading:17026720-Oncogene Proteins, Viral, pubmed-meshheading:17026720-Papillomavirus E7 Proteins, pubmed-meshheading:17026720-Papillomavirus Infections, pubmed-meshheading:17026720-T-Lymphocyte Subsets, pubmed-meshheading:17026720-Uterine Cervical Neoplasms
pubmed:year
2006
pubmed:articleTitle
Comparison of cervical and blood T-cell responses to human papillomavirus-16 in women with human papillomavirus-associated cervical intraepithelial neoplasia.
pubmed:affiliation
Division of Medical Virology, Department Clinical Laboratory Science and Institute of Infectious Disease and Molecular Medicine, Cape Town, South Africa. joann@curie.uct.ac
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't