Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-1-17
pubmed:abstractText
Tyrosine nitration is a nitric oxide-derived post-translational modification of proteins. Elevated levels of specific plasma proteins modified by tyrosine nitration have been detected during acute and chronic inflammatory conditions, including acute lung injury (ALI). In the present study we examined whether circulating immunoglobulins against nitrated proteins are present in the plasma of subjects with clinically documented ALI. Affinity chromatography using covalently linked 3-nitrotyrosine was employed to identify plasma proteins that bind to this unusual amino acid. Western blotting and liquid chromatography-tandem mass spectrometry of in-gel digested protein bands revealed that the major proteins eluted from the affinity column were IgM and IgG. An enzyme-linked immunosorbent assay (ELISA) based on competition of horseradish peroxidase-derivatized 3-nitrotyrosine binding to plasma with unlabeled 3-nitrotyrosine was developed and validated. Using this ELISA, the levels of immunoglobulins that recognize 3-nitrotyrosine were significantly higher in the plasma of subjects with ALI compared with both normal control subjects and subjects with major trauma who did not develop ALI (0.36+/- 0.14 versus 0.03 +/- 0.05, and 0.25 +/- 0.15; P < 0.001 and P = 0.006, respectively). These data indicate that tyrosine-nitrated proteins induce the production of specific immunoglobulins during acute phase response and inflammation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-10080827, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-10471637, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-10507592, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-10735360, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-10781426, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11062131, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11179123, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11207999, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11208643, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11243306, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11588459, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11728809, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-11981321, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12175814, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12394964, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12411948, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12663342, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12690103, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12847213, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12851409, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-12873048, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-14729508, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-15314690, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-16000355, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-16716892, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-1959406, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-3339667, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-7509706, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-7989597, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-8192861, http://linkedlifedata.com/resource/pubmed/commentcorrection/17023686-9504573
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
152-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Identification of immunoglobulins that recognize 3-nitrotyrosine in patients with acute lung injury after major trauma.
pubmed:affiliation
Stokes Research Institute, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, PA 19104-4318, USA. thomson@email.chop.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural