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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2006-10-5
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pubmed:abstractText |
Sapoviruses (SVs) are an important cause of acute pediatric gastroenteritis. Due to the lack of appropriate diagnostic methods, the epidemiology of SV-associated illness remains poorly understood. Baculovirus and Escherichia coli expression systems were evaluated for the development of antibody detection enzyme immunoassays (EIA). Age-related antibody prevalence in children was studied using the new EIA. Because of the low yield of the baculovirus system, the E. coli-expressed SV capsid proteins were used to develop the EIA. The antigenic specificities of the E. coli-expressed SV capsid proteins were demonstrated using hyperimmune antisera raised in animals and sera collected from patients. A high prevalence (>90%) of antibodies to both SV (strain Mex340) and norovirus (strain VA387) was observed in children involved in a birth cohort at 20 to 24 months of age; however, at 1 to 3 months of age, <25% of the children possessed anti-SV antibodies versus >90% with anti-NV antibodies. The E. coli-derived SV proteins are an excellent source of antigens for the EIA. SV infection is common in the first 2 years of life. The low prevalence of maternal antibodies detected in Mexican children against SVs in this study is unique and needs to be addressed in future studies.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10096931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10204699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10353514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10568770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10686033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10804140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10804145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-10804148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-11002251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-11035949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-11148001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-11283075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-11710959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-11855626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-12459479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-12696113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-12791840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-12791850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-12825167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-1328679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-1383265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-14718622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-14738976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15122801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15221533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15449145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15484274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15648058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15695660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15708314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15737920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-15849053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-16254337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-2177224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-232140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-2993432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-3001130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-3182991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-4117963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-6270278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-7710362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-8158109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-8608642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-8928224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-9672617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-9774557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17021096-9877192
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3674-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17021096-Aging,
pubmed-meshheading:17021096-Antibodies, Viral,
pubmed-meshheading:17021096-Baculoviridae,
pubmed-meshheading:17021096-Caliciviridae Infections,
pubmed-meshheading:17021096-Child, Preschool,
pubmed-meshheading:17021096-Escherichia coli,
pubmed-meshheading:17021096-Gene Expression Regulation, Viral,
pubmed-meshheading:17021096-Humans,
pubmed-meshheading:17021096-Immunoenzyme Techniques,
pubmed-meshheading:17021096-Infant,
pubmed-meshheading:17021096-Mexico,
pubmed-meshheading:17021096-Sapovirus,
pubmed-meshheading:17021096-Seroepidemiologic Studies
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pubmed:year |
2006
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pubmed:articleTitle |
Development of an enzyme immunoassay for detection of sapovirus-specific antibodies and its application in a study of seroprevalence in children.
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pubmed:affiliation |
Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA. Tibor.Farkas@cchmc.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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