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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1991-2-4
|
| pubmed:abstractText |
Amino acid sequences inducing neutralizing antibodies to HIV-1 were sought. Murine monoclonal antibodies (MAbs) were characterized by their reactivity with the envelope precursor gp160 or the Escherichia coli recombinant DNA products pB1 and pE3 representing the carboxy- and amino-terminal halves of mature envelope gp120. Fine mapping of the MAb determinants was performed using defined 15-mer synthetic peptides spanning the entire envelope gp120 region of HIV-1. One group of MAbs recognizes epitopes (amino acids 304-323) occurring in a small region with variable and conserved amino acid sequences of gp120. These MAbs mediate neutralization of the HIV-1 strain HTLV-IIIB (HIV-1IIIB) which was used for immunization. Nine out of 11 primary HIV-1 isolates were neutralized well or moderately well. In addition, prominent serological reactivity was noted with peptide sequences of strains of various European or American origins, but not with two HIV-1 strains of African origin. The cross-reactivity contrasts with previously described type-specific reactions to other sequences of this region. The reactivity to the short conserved site GPGR with its flanking amino acids may explain the broad sequence cross-reactivity seen with our neutralizing MAbs. Two other MAbs recognize conserved epitopes (amino acids 79-103) situated in the amino-terminal region of gp120. These MAbs did not neutralize HIV-1IIIB.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp160,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0269-9370
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
953-60
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1702001-Amino Acid Sequence,
pubmed-meshheading:1702001-Animals,
pubmed-meshheading:1702001-Antibodies, Monoclonal,
pubmed-meshheading:1702001-Blotting, Western,
pubmed-meshheading:1702001-Cross Reactions,
pubmed-meshheading:1702001-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1702001-Epitopes,
pubmed-meshheading:1702001-Gene Products, env,
pubmed-meshheading:1702001-HIV Antibodies,
pubmed-meshheading:1702001-HIV Envelope Protein gp120,
pubmed-meshheading:1702001-HIV Envelope Protein gp160,
pubmed-meshheading:1702001-HIV-1,
pubmed-meshheading:1702001-Immunization,
pubmed-meshheading:1702001-Mice,
pubmed-meshheading:1702001-Molecular Sequence Data,
pubmed-meshheading:1702001-Neutralization Tests,
pubmed-meshheading:1702001-Peptide Mapping,
pubmed-meshheading:1702001-Protein Precursors,
pubmed-meshheading:1702001-Recombinant Proteins
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Neutralizing cross-reactive and non-neutralizing monoclonal antibodies to HIV-1 gp120.
|
| pubmed:affiliation |
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Biomedical Center, Uppsala, Sweden.
|
| pubmed:publicationType |
Journal Article
|