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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-1-30
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pubmed:abstractText |
We have isolated a murine myeloid precursor cell line (FDC-P1/MAC) that simultaneously expresses receptors for multi-CSF, GM-CSF, and M-CSF (c-fms protooncogene). FDC-P1/MAC cells express high levels of c-fms mRNA and protein when grown in M-CSF, whereas growth in multi-CSF or GM-CSF caused a dramatic reduction of c-fms glycoprotein and mRNA. Nuclear run-off assays demonstrated that c-fms transcription was not growth factor dependent and the regulation occurred posttranscriptionally. Factor switching experiments have shown that both multi-CSF and GM-CSF act dominantly and in a factor concentration dependent manner to suppress c-fms expression. In vitro agar assays of bone marrow cells grown in the presence of GM-CSF and M-CSF, individually and in combination, support the concept that GM-CSF can act dominantly to prevent monocyte/macrophage development. These results suggest that GM-CSF and multi-CSF can suppress development along the monocyte/macrophage lineage and offer a simple stochastic mechanism governing myeloid lineage restriction.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
63
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pubmed:geneSymbol |
c-fms
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1073-83
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1701692-Animals,
pubmed-meshheading:1701692-Blotting, Northern,
pubmed-meshheading:1701692-Cell Line,
pubmed-meshheading:1701692-Cell Nucleus,
pubmed-meshheading:1701692-Down-Regulation,
pubmed-meshheading:1701692-Flow Cytometry,
pubmed-meshheading:1701692-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1701692-Hematopoiesis,
pubmed-meshheading:1701692-Hematopoietic Stem Cells,
pubmed-meshheading:1701692-Immunoblotting,
pubmed-meshheading:1701692-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1701692-Mice,
pubmed-meshheading:1701692-Poly A,
pubmed-meshheading:1701692-Proto-Oncogenes,
pubmed-meshheading:1701692-RNA,
pubmed-meshheading:1701692-RNA, Messenger,
pubmed-meshheading:1701692-RNA Processing, Post-Transcriptional,
pubmed-meshheading:1701692-Receptor, Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1701692-Suppression, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Expression of the M-CSF receptor is controlled posttranscriptionally by the dominant actions of GM-CSF or multi-CSF.
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pubmed:affiliation |
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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