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rdf:type |
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lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0026473,
umls-concept:C0027651,
umls-concept:C0030956,
umls-concept:C0035015,
umls-concept:C0039195,
umls-concept:C0086418,
umls-concept:C0205245,
umls-concept:C0205263,
umls-concept:C0205369,
umls-concept:C1441547,
umls-concept:C1548437,
umls-concept:C1882923
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pubmed:issue |
5
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pubmed:dateCreated |
2006-10-3
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pubmed:abstractText |
Dendritic cells (DCs) are powerful antigen-presenting cells (APCs), that have so far been applied for cancer specific immunotherapy. Recent results suggest that matured DCs derived from human monocytes have a significant impact on the outcome of vaccination. The conventional generation of mature DCs from human monocytes in vitro has been reported to require 5 days for differentiation with granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-4 and 2 days for stimulation. We herein report a new strategy for the functional maturation of monocyte-derived DCs within only 2 days of in vitro culture and the induction of specific cytotoxic T lymphocytes (CTLs) to tumor rejection peptide. The monocytes were incubated for 1 day with GM-CSF and IL-4, followed by activation with a bacterial product, OK-432 and prostaglandin E2 (PGE2) for another 1 day (rapid DC). Rapid DC expressed mature DC surface markers as well as chemokine receptor 7 and secreted Th1-type cytokines. The DCs generated in this study mobilized Ca2+ in response to CCL21/6Ckine and SDF-1, but only marginally did so to Mip-1alpha. Moreover, when rapid DC were compared with mature conventional 7-day DCs, they were equally potent in inducing specific CTLs in vitro. These results indicate that the rapid DC is as effective as the monocyte-derived conventional DCs. The rapid DC would be a potentially useful new cancer-specific immunotherapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/CCL21 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CXCL12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL21,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A28 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/MAGEA3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Picibanil
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1019-6439
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1263-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17016660-Antigens, Neoplasm,
pubmed-meshheading:17016660-Calcium,
pubmed-meshheading:17016660-Cell Culture Techniques,
pubmed-meshheading:17016660-Chemokine CCL21,
pubmed-meshheading:17016660-Chemokine CXCL12,
pubmed-meshheading:17016660-Chemokines, CC,
pubmed-meshheading:17016660-Chemokines, CXC,
pubmed-meshheading:17016660-Coculture Techniques,
pubmed-meshheading:17016660-Cytokines,
pubmed-meshheading:17016660-Dendritic Cells,
pubmed-meshheading:17016660-Dinoprostone,
pubmed-meshheading:17016660-HLA-A Antigens,
pubmed-meshheading:17016660-Humans,
pubmed-meshheading:17016660-Immunotherapy, Adoptive,
pubmed-meshheading:17016660-Interferon-gamma,
pubmed-meshheading:17016660-Interleukin-12,
pubmed-meshheading:17016660-Monocytes,
pubmed-meshheading:17016660-Neoplasm Proteins,
pubmed-meshheading:17016660-Peptides,
pubmed-meshheading:17016660-Picibanil,
pubmed-meshheading:17016660-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:17016660-Th1 Cells
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pubmed:year |
2006
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pubmed:articleTitle |
Efficient induction of specific cytotoxic T lymphocytes to tumor rejection peptide using functional matured 2 day-cultured dendritic cells derived from human monocytes.
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pubmed:affiliation |
Division of Molecular and Surgical Oncology, Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Beppu 874-0838, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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