Linked Life Data

17015276

Source:http://linkedlifedata.com/resource/pubmed/id/17015276

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2006-10-3
pubmed:abstractText
Dichlorofluorescin (DCFH), a widely used fluorescent probe for reactive oxygen species (ROS) was decomposed completely and generated two distinct fluorescent products by photo-irradiation at 254 nm for 30 min. In the previous study, we had shown that one was dichlorofluorescein (DCF), a well known oxidized product of DCFH. In this study we investigated the other product and identified it as monochlorofluorescein (MCF) by 1H-NMR and fast atom bombardment/mass spectrum (FAB/MS) analyses. MCF was generated by photo-irradiation, but not by ROS. On the other hand, DCF was produced by both photo-irradiation and ROS. MCF showed similar fluorescent emission spectrum to DCF, however, its fluorescence intensity was more than that of DCF. The kinetic study suggested that MCF was not generated from DCF but from monochlorofluorescin, which might be generated from DCFH by photo-irradiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1071-5762
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
959-65
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Generation of a novel fluorescent product, monochlorofluorescein from dichlorofluorescin by photo-irradiationdagger.
pubmed:affiliation
Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Division of Applied Biological Sciences, 4-3-11 Takeda, Kofu, Japan.
pubmed:publicationType
Journal Article