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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
2006-11-13
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pubmed:abstractText |
Metarhodopsin-II is the light-excited form of rhodopsin that triggers G protein function. Metarhodopsin-II is stabilized when the N-terminus of the carboxyl (340-350) tail peptide of the alpha-subunit of transducin (Gtalpha) is crosslinked to rhodopsin cysteine 140 or the 340-350 peptide C-terminus of Gtalpha is crosslinked to rhodopsin cysteine 316. When the N-terminus of the peptide is coupled to C316 the MI<-->MII equilibrium is not affected. The evidence suggests that the N-terminus of the 340-350 region of Gtalpha is located near C140 when transducin stabilizes metarhodopsin-II and alternative explanations are suggested for the effectiveness of the 340-350 Gtalpha tail peptide when bound to C316.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0042-6989
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4547-55
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17014882-Animals,
pubmed-meshheading:17014882-Cattle,
pubmed-meshheading:17014882-Cross-Linking Reagents,
pubmed-meshheading:17014882-Cysteine,
pubmed-meshheading:17014882-Dark Adaptation,
pubmed-meshheading:17014882-GTP-Binding Protein alpha Subunits,
pubmed-meshheading:17014882-Peptide Fragments,
pubmed-meshheading:17014882-Protein Binding,
pubmed-meshheading:17014882-Receptors, G-Protein-Coupled,
pubmed-meshheading:17014882-Rhodopsin,
pubmed-meshheading:17014882-Rod Cell Outer Segment,
pubmed-meshheading:17014882-Spectrum Analysis
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pubmed:year |
2006
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pubmed:articleTitle |
Metarhodopsin-II stabilization by crosslinked Gtalpha C-terminal peptides and implications for the mechanism of GPCR-G protein coupling.
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pubmed:affiliation |
Department of Microbiology, Montana State University, Bozeman, MT 59717, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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