|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
23
|
|
pubmed:dateCreated |
1991-1-16
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilobase sequence that was flanked by direct repeats of a 26-base-pair sequence found in the K-12 chromosomal DNA. This suggests that the 34-kilobase element was probably integrated into the E. coli genome by a mechanism related to transposition or phage integration. In the 34-kilobase sequence an open reading frame of 285 residues was found, which displays 44% sequence identity with the E. coli Dam methylase. Interestingly, there are three GATC sequences, the site of Dam methylation, in the promoter region of the gene for reverse transcriptase.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-1692831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2180909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2446773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2465092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2466332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2466572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2466573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2471077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2667217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2704042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2842065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-2992361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-3000598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-3028246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-3038334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-3071260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-3806670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-4568763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-4600143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-6295879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-6300769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701261-6546423
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
87
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
9454-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:1701261-Amino Acid Sequence,
pubmed-meshheading:1701261-Base Sequence,
pubmed-meshheading:1701261-Chromosome Mapping,
pubmed-meshheading:1701261-Chromosomes, Bacterial,
pubmed-meshheading:1701261-DNA, Single-Stranded,
pubmed-meshheading:1701261-DNA Transposable Elements,
pubmed-meshheading:1701261-Escherichia coli,
pubmed-meshheading:1701261-Escherichia coli Proteins,
pubmed-meshheading:1701261-Methyltransferases,
pubmed-meshheading:1701261-Molecular Sequence Data,
pubmed-meshheading:1701261-RNA-Directed DNA Polymerase,
pubmed-meshheading:1701261-Restriction Mapping,
pubmed-meshheading:1701261-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1701261-Site-Specific DNA-Methyltransferase (Adenine-Specific)
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Retron for the 67-base multicopy single-stranded DNA from Escherichia coli: a potential transposable element encoding both reverse transcriptase and Dam methylase functions.
|
|
pubmed:affiliation |
Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
